Chloroplast Research in Arabidopsis

Screening or Selection for Chloroplast Biogenesis Mutants of Arabidopsis, Following Chemical or Insertional Mutagenesis.- Analysis of Plastid Number, Size, and Distribution in Arabidopsis Plants by Light and Fluorescence Microscopy.- Immunofluorescence Microscopy for Localization of Arabidopsis Chloroplast Proteins.- Transient Expression and Analysis of Chloroplast Proteins in Arabidopsis Protoplasts.- Visualisation of Stromules on Arabidopsis Plastids.- Analysis of Chloroplast Movement and Relocation in Arabidopsis.- Studying Starch Content and Sedimentation of Amyloplast Statoliths In Arabidopsis Roots.- Studying Arabidopsis Chloroplast Structural Organization Using Transmission Electron Microscopy.- Transplastomics in Arabidopsis: Progress Towards Developing an Efficient Method.- Isolation, Quantification, and Analysis of Chloroplast DNA.- Measurement of Transcription Rates in Arabidopsis Chloroplasts.- Studying the Structure and Processing of Chloroplast Transcripts.- In Vitro RNA Binding Assay for Studying Trans-Factors for RNA Editing in Chloroplasts.- Studying Translation in Arabidopsis Chloroplasts.- Studying Proteases and Protein Turnover in Arabidopsis Chloroplasts.- In Silico Methods for the Identification of Organellar and Suborganellar Targeting Peptides in Arabidopsis Chloroplast Proteins and for Predicting the Topology of Membrane Proteins.- Rapid Isolation of Arabidopsis Chloroplasts and Their Use for In Vitro Protein Import Assays.- Energetic Manipulation of Chloroplast Protein Import and the Use of Chemical Cross-Linkers to Map Protein-Protein Interactions.- Isolation of Arabidopsis Thylakoid Membranes and Their Use for In Vitro Protein Insertion or Transport Assays.- Determining the Location of an Arabidopsis Chloroplast Protein Using In Vitro Import Followed by Fractionation and Alkaline Extraction.- Studying Arabidopsis Envelope Protein Localization and Topology Using Thermolysin and Trypsin Proteases.