Chromatin Remodeling

Strain Construction and Screening Methods for a Yeast Histone H3/H4 Mutant Library.- Measuring Dynamic Changes in Histone Modifications and Nucleosome Density during Activated Transcription in Budding Yeast.- Monitoring the Effects of Chromatin Remodelers on Long-Range Interactions In Vivo.- Measuring Nucleosome Occupancy In Vivo by Micrococcal Nuclease.- Analysis of Nucleosome Positioning Using a Nucleosome-Scanning Assay (NuSA).- Assaying Chromatin Structure and Remodeling by Restriction Enzyme Accessibility.- Generation of DNA Circles in Yeast by Inducible Site-Specific Recombination.- An Efficient Purification System for Native Minichromosome from S. cerevisiae.- Simultaneous, Single-Molecule Detection of Endogenous C-5 DNA Methylation and Chromatin Accessibility Using MAPit.- Analysis of Stable and Transient Protein-Protein Interactions.- Monitoring Dynamic Binding of Chromatin Proteins In Vivo by Fluorescence Recovery after Photobleaching (FRAP).- Monitoring Dynamic Binding of Chromatin Proteins In Vivo by Fluorescence Correlation Spectroscopy (FCS) and Temporal Image Correlation Spectroscopy (TICS).- Analysis of Chromatin Structure in Plant Cells.- Analysis of Histones and Histone Variants in Plants.- Reconstitution of Modified Chromatin Templates for In Vitro Functional Assays.- A Defined In Vitro System to Study ATP-Dependent Remodeling of Short Chromatin Fibers.- In Vitro Reconstitution of In Vivo-Like Nucleosome Positioning on Yeast DNA.- Activator-Dependent Acetylation of Chromatin Model Systems.- Mapping Assembly-Favored and Remodeled Nucleosome Positions on Polynucleosomal Templates.- Analysis of Changes in Nucleosome Conformation Using Fluorescence Resonance Energy Transfer (FRET).- Preparation of Nucleosomes Containing a Specific H2A-H2A Crosslink Forming a DNA-Constraining Loop Structure.- Sulfyhydryl Reactive Site-Directed Crosslinking as a Method for Probing the Tetrameric Structure of Histones H3 and H4.- Genomic Approaches for Determining

From the reviews:"This is an outstanding and timely collection of methods concerning how one can investigate chromatin remodelling. ... There are detailed laboratory procedures and notes attached to all chapters, and this enable readers to use the volume as a laboratory manual. ... The editor, Randall Morse, has to be congratulated on producing such an excellent book; a book that I recommend most highly to researchers in this arena." (Raymond Waters, Human Genetics, Vol. 131, 2012)