Colorectal Cancer

Microdissection of Histological Sections: Manual and Laser Capture Microdissection Techniques Christopher A. Moskaluk Isolation of a Purified Epithelial Cell Population from Human Colon James K. Roche Xenografting Human Colon Cancers Jeffrey C. Harper, Reid B. Adams, and Steven M. Powell Comparative Genomic Hybridization Technique Wa'el El-Rifai and Sakari Knuutila Fluorescence In Situ Hybridization: Application in Cancer Research and Clinical Diagnostics Svetlana D. Pack and Zhengping Zhuang Microsatellite Analysis to Assess Chromosome 18q Status in Colorectal Cancer Jin Jen The SURF Technique: Selective Genetic Analysis of Microscopic Tissue Heterogeneity Darryl Shibata Microsatellite Instability Testing Yann R. Parc and Kevin C. Halling Immunohistochemical Analysis for hMLH1 and hMSH2 Expression in Colorectal Cancer Kevin C. Halling and Patrick C. Roche Mutation Detection in Colorectal Cancers: Direct Sequencing of DNA Mismatch Repair Genes Julie M. Cunningham, David J. Tester, and Stephen N. Thibodeau Mutation Detection in the TGF-b Receptors William M. Grady, Lois L. Myeroff, Hongmei He, and Sanford Markowitz Direct Analysis for Familial Adenomatous Polyposis Mutations Steven M. Powell I1307K Mutation Detection by Allele-Specific PCR in Familial Colorectal Cancer Perry S. Chan and Steven L. Gersen Microsatellite Analysis of the Insulin-Like Growth Factor II Receptor in Colorectal Carcinomas Rhonda F. Souza and Stephen J. Meltzer Molecular Detection of Smad2/Smad4 Alterations in Colorectal Tumors Sam Thiagalingam Direct Sequencing for Juvenile Polyposis Gene SMAD4/DPC4 Mutations Lauri A. Aaltonen and Stina Roth Direct Sequencing for Peutz-Jehgers Gene LKB1 (STK11) Mutations Lauri A. Aaltonen and Egle Avizienyte Direct Sequencing for Cowden Syndrome Gene PTEN (MMAC1) Mutations Lauri A. Aaltonen, Stina Roth, and Charis Eng Genetic Analysis Using Microarrays Wa'el El-Rifai and Sakari Knuutila Cyclooxygenase-2 (COX-2) Protein Expression by Western Blotting Hossam M.

This is quite interesting and a valuable volume. It aims to instruct investigators in the key genetic, cellular and molecular biological methods for analyzing colorectal tumors. It's quite precise in its description of these techniques and, as the preface says, The focused techniques and assays are described in sufficient detail to allow researchers to start an experiment on colon tumors and proceed from beginning to end as if the expert in the field who has performed these studies were guiding them at the bench. A large amount of practical information is packed into one small volume and this volume is ideal for students and fellows who wish to expand their knowledge in this area and have a ready volume reference in developing new techniques The volume is also good value for those of us who don't get into the lab as it clarifies many of the techniques which are used and helps the non-involved reader to understand the terminology and techniques used in this important area of cancer research. - Cancer Forum ...Dr. Saltz has produced as concise a presentation as possible within the framework of the widely scattered disciplines searching the complexity of diagnostic and therapeutic problems occurring among patients with colorectal cancer. Comprehensive Therapy