Culture of Human Embryonic Stem Cells
Methods and Techniques
(Sprache: Englisch)
A handbook of the practical aspects of working with human embryonic stem cells from their derivation to the development of clinical applications. The chapters would start with a brief review and then give practical details for experimental analysis and...
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A handbook of the practical aspects of working with human embryonic stem cells from their derivation to the development of clinical applications. The chapters would start with a brief review and then give practical details for experimental analysis and research. It would also cover ethical issues and requirements for clinical developments.
Klappentext zu „Culture of Human Embryonic Stem Cells “
A handbook of the practical aspects of working with human embryonic stem cells from their derivation to the development of clinical applications. The chapters would start with a brief review and then give practical details for experimental analysis and research. It would also cover ethical issues and requirements for clinical developments.
A handbook of the practical aspects of working with human embryonic stem cells from their derivation to the development of clinical applications. The chapters would start with a brief review and then give practical details for experimental analysis and research. It would also cover ethical issues and requirements for clinical developments.
Inhaltsverzeichnis zu „Culture of Human Embryonic Stem Cells “
From the contents:Background to embryonic stem cells.- Regulatory and ethical issues.- Setting up a lab.- Establishing a hES research programme.- Embryonic stem cell derivation.- Principles of hES cell culture.- Cryopreservation of cell lines.- Feeder-free techniques.- Advanced culture techniques for hES cells.- Avoiding culture pitfalls.- Methods of differentiation.- Differentiation Protocols.- Analysis of differentiation.- Immunofluorescent labeling for surface antigens and FACS analysis.- Flow Cytometry.- Transfection.- Molecular profiling of hES cells.- Quality control and assurance.
Bibliographische Angaben
- 2011, 250 Seiten, Maße: 15,5 x 23,5 cm, Kartoniert (TB), Englisch
- Ed.: Moore, Harry
- Herausgegeben: Harry Moore
- Verlag: Springer, Berlin
- ISBN-10: 1603275398
- ISBN-13: 9781603275392
Sprache:
Englisch
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