Development of a Collagen Gel Sandwich Hepatocyte Bioreactor
In Vitro Detection of Hepatotoxicity Caused by Drugs and Chemicals
(Sprache: Englisch)
Understanding the hepatotoxicity of drugs is essential for progress in academic and industrial research. Studying hepatotoxicity in vitro is challenging because hepatocytes, the metabolically active cells of the liver, are very difficult to maintain in...
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Understanding the hepatotoxicity of drugs is essential for progress in academic and industrial research. Studying hepatotoxicity in vitro is challenging because hepatocytes, the metabolically active cells of the liver, are very difficult to maintain in culture. It has been observed that culturing hepatocytes between two layers of collagen maintains in vivo-like morphology and also many drug metabolizing enzymes for weeks. In spite of the research examining drug metabolism in collagen sandwiches, there is limited data regarding the applicability of this system to hepatotoxicity studies. We cultured primary rat hepatocytes in the collagen sandwich configuration and optimized this system for long-term hepatotoxicity studies. In addition, we also examined the conditioned medium from the hepatocytes to investigate the secreted protein profile, which could potentially be used to find toxicity biomarkers. Our proteomics analysis combined gel electrophoresis with Qstar LC-MS/MS, and we were able to identify 493 proteins. In summary, we have shown that the collagen sandwich is a suitable system for hepatotoxicity studies as well as proteomics analysis.
Autoren-Porträt von Dora Farkas
Farkas, DoraDora Farkas, Ph.D., completed both her Bachelor's degree inChemical Engineering and her Ph.D. in Toxicology at theMassachusetts Institute of Technology. After she earned herPh.D. she worked as a postdoctoral fellow in the Department ofPharmacology and Experimental Therapeutics at Tufts University inBoston.
Bibliographische Angaben
- Autor: Dora Farkas
- 2010, 156 Seiten, Maße: 22 cm, Kartoniert (TB), Englisch
- Verlag: LAP Lambert Academic Publishing
- ISBN-10: 3838350162
- ISBN-13: 9783838350165
Sprache:
Englisch
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