Digital Holography Microscopy applications

This book describes general and robust algorithms
that are devoted to automate the analysis process in
the 3D space and in time of dynamic objects present
in a volume studied by a specific microscope that
permits to record images containing their 3D
information. This imaging system using a coherent
light records not only the intensity information of
the light, but also the direction of propagation for
each pixel in the image. Together with a numerical
model for coherent light diffraction, it is possible
to generate numerically other images parallel to the
recorded plane and as a consequence to refocus
numerically objects. The acquisition time of 3D data
is as a consequence only limited by the acquisition
rate of the CCD camera of in the imaging system.
The extraction of 3D information is performed by
algorithm applied on the recorded data. The studied
objects are either of biological nature or latex
particles. A focus criterion to determine the best
focus planeof an object is proposed. From the
knowledge of the 3D position of objects, several
analyses are proposed like shape or object
trajectories analysis in time and in 3D.