DNA Methylation
Methods and Protocols
(Sprache: Englisch)
Over the past few years, DNA methylation technologies and our knowledge of DNA methylation patterns have been advancing at a breathtaking pace. Due to this fact, DNA Methylation: Methods and Protocols, Second Edition completely revises, updates, and expands...
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Over the past few years, DNA methylation technologies and our knowledge of DNA methylation patterns have been advancing at a breathtaking pace. Due to this fact, DNA Methylation: Methods and Protocols, Second Edition completely revises, updates, and expands upon the popular first edition with the most current novel techniques, easier to use and more refined by the tested experience of leading experts. This revision reflects contemporary study of the subject: the analysis of gene-specific DNA methylation patterns has been complemented by genome-wide approaches, and epigenomics takes a central place. Written in the highly successful Methods in Molecular Biology(TM) series format, the chapters in this volume present brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes which highlight tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, DNA Methylation: Methods and Protocols, Second Edition is the perfect resource for scientists who wish to further our understanding of the molecular processes that determine the genomic DNA methylation landscape and for those who aim to identify and implement DNA methylation-based biomarkers for clinical and diagnostic applications.
Klappentext zu „DNA Methylation “
Over the past few years, DNA methylation technologies and our knowledge of DNA methylation patterns have been advancing at a breathtaking pace. Due to this fact, DNA Methylation: Methods and Protocols, Second Edition completely revises, updates, and expands upon the popular first edition with the most current novel techniques, easier to use and more refined by the tested experience of leading experts. This revision reflects contemporary study of the subject: the analysis of gene-specific DNA methylation patterns has been complemented by genome-wide approaches, and epigenomics takes a central place. Written in the highly successful Methods in Molecular Biology(TM) series format, the chapters in this volume present brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes which highlight tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, DNA Methylation: Methods and Protocols, Second Edition is the perfect resource for scientists who wish to further our understanding of the molecular processes that determine the genomic DNA methylation landscape and for those who aim to identify and implement DNA methylation-based biomarkers for clinical and diagnostic applications.
DNA methylation technologies and knowledge of DNA methylation patterns have been advancing at breakneck speed. This expanded, updated and revised edition incorporates all the new information in the field.
Inhaltsverzeichnis zu „DNA Methylation “
Part I: Introduction 1. DNA Methylation: An Introduction to the Biology and the Disease-Associated Changes of a Promising Biomarker Jörg Tost
Part II: Global Methylation Levels
2. Quantification of Global DNA Methylation by Capillary Electrophoresis and Mass Spectrometry María Berdasco, Mario F. Fraga, and Manel Esteller
3. Methyl Group Acceptance Assay for the Determination of Global DNA Methylation Levels Kenneth P. Nephew, Curt Balch, and David G. Skalnik
Part III: Methods for Whole Genome Analysis of DNA Methylation Patterns
4. Immunodetection Array Johannes Pröll, Christian Wechselberger, Mathilde Födermayr, Otto Zach, and Dieter Lutz
5. Methylated DNA Immunoprecipitation (MeDIP) Fabio Mohn, Dirk Schübeler, and Tim-Christoph Roloff
6. The MIRA Method for DNA Methylation Analysis Tibor A. Rauch and Gerd P. Pfeifer
7. The HELP Assay Mayumi Oda and John M. Greally
8. Differential Methylation Hybridization: Profiling DNA Methylation with a High Density CpG Island Microarray Pearlly S. Yan, Dustin Potter, Daniel E Deatherage, Shili Lin, and Tim H.-M. Huang
9. Analysis of DNA Methylation by Amplification of InterMethylated Sites (AIMS) Mireira Jordà, Jairo Rodriguez, Jordi Frigola, and Miguel A. Peinado
10. Methylation-Sensitive Representational Difference Analysis (MS-RDA) Toshikazu Ushijima and Satoshi Yamashita
11. Restriction Landmark Genomic Scanning: Analysis of CpG Islands in Genomes by 2D Gel Electrophoresis Joseph F. Costello, Dominic J. Smiraglia, Chibo Hong, and Christoph Plass
12. GoldenGate® Assay for DNA Methylation Profiling Marina Bibikova and Jian-Bing Fan
13. 5'-Azacytidine Expression Arrays Paul Cairns
Part IV: Gene Specific Methylation Analysis
14. DNA Methylation Analysis by Bisulfite Conversion, Cloning, and Sequencing of Individual Clones Yingying Zhang, Christian Rohde, Sascha Tierling, Heinrich Stamerjohanns, Richard Reinhardt, Jörn Walter, and Albert Jeltsch
15.
... mehr
Identification and Quantification of Differentially Methylated Loci by the PyrosequencingTM Technology Emelyne Dejeux , Hafida El abdalaoui, Ivo Glynne Gut, and Jörg Tost
16. Mass Spectrometric Analysis of Cytosine Methylation by Base-Specific Cleavage and Primer Extension Methods Dirk van den Boom and Mathias Ehrich
17. Melting Curve Assays for DNA Methylation Analysis Tomasz K. Wojdacz and Alexander Dobrovic
18. Methylation SNaPshot: A Method for Quantification of Site Specific DNA Methylation Levels Zachary Kaminsky and Arturas Petronis
19. Bio-COBRA: Absolute Quantification of DNA Methylation in Electrofluidics Chips Romulo Martin Brena and Christoph Plass
20. Restriction Digestion and Real-time PCR (qAMP) Christopher C. Oakes, Sophie La Salle, Jacquetta M. Trasler, and Bernard Robaire
21. MethylQuant : A Real-Time PCR Based Method to Quantify DNA Methylation at Single Specific Cytosines Claire Dugast-Darzacq and Thierry Grange
22. Methylation-Specific PCR Julien D. F. Licchesi and James G. Herman
23. MethyLight Mihaela Campan, Daniel J. Weisenberger, Binh Trinh, and Peter W. Laird
24. Quantification of Methylated DNA by HeavyMethyl Duplex PCR Jürgen Distler
Part V: Special Applications
25. Analysis of Methylated Circulating DNA in Cancer Patients' Blood Eiji Sunami, Anh-Thu Vu, Sandy L. Nguyen, and Dave S.B. Hoon
26. Prevention of PCR Cross Contamination by UNG Treatment of Bisulfite Treated DNA Reimo Tetzner
27. Profiling DNA Methylation from Small Amounts of Genomic DNA Starting Material: Efficient Sodium Bisulfite Conversion and Subsequent Whole-Genome Amplification Jonathan Mill and Arturas Petronis
16. Mass Spectrometric Analysis of Cytosine Methylation by Base-Specific Cleavage and Primer Extension Methods Dirk van den Boom and Mathias Ehrich
17. Melting Curve Assays for DNA Methylation Analysis Tomasz K. Wojdacz and Alexander Dobrovic
18. Methylation SNaPshot: A Method for Quantification of Site Specific DNA Methylation Levels Zachary Kaminsky and Arturas Petronis
19. Bio-COBRA: Absolute Quantification of DNA Methylation in Electrofluidics Chips Romulo Martin Brena and Christoph Plass
20. Restriction Digestion and Real-time PCR (qAMP) Christopher C. Oakes, Sophie La Salle, Jacquetta M. Trasler, and Bernard Robaire
21. MethylQuant : A Real-Time PCR Based Method to Quantify DNA Methylation at Single Specific Cytosines Claire Dugast-Darzacq and Thierry Grange
22. Methylation-Specific PCR Julien D. F. Licchesi and James G. Herman
23. MethyLight Mihaela Campan, Daniel J. Weisenberger, Binh Trinh, and Peter W. Laird
24. Quantification of Methylated DNA by HeavyMethyl Duplex PCR Jürgen Distler
Part V: Special Applications
25. Analysis of Methylated Circulating DNA in Cancer Patients' Blood Eiji Sunami, Anh-Thu Vu, Sandy L. Nguyen, and Dave S.B. Hoon
26. Prevention of PCR Cross Contamination by UNG Treatment of Bisulfite Treated DNA Reimo Tetzner
27. Profiling DNA Methylation from Small Amounts of Genomic DNA Starting Material: Efficient Sodium Bisulfite Conversion and Subsequent Whole-Genome Amplification Jonathan Mill and Arturas Petronis
... weniger
Bibliographische Angaben
- 2010, 2nd Edition. Softcover version of original hardcover edition 2009, XVI, 392 Seiten, Maße: 19,3 x 26 cm, Kartoniert (TB), Englisch
- Herausgegeben: Jörg Tost
- Verlag: Springer, Berlin
- ISBN-10: 1617379387
- ISBN-13: 9781617379383
Sprache:
Englisch
Pressezitat
From the reviews of the second edition: "The 2nd edition ... resulted in an extraordinarly helpful tool for lab practice and for developing multiples approaches in order to successfully perform these type of analysis. ... authors made each chapter a valuable tool for beginners and senior investigators who want to explore DNA methylation analysis in their biological systems. ... used for advanced researchers in order to complement their work with other methodologies. ... This is definitively a volume that must be present at each lab working in this cutting-edge field ... ." (Marcia Manterola, European Journal of Histochemistry, October, 2009)
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