DNA Repair Protocols

Mammalian Cell Extracts.- 25 Nucleotide Excision Repair in Saccharomyces cerevisiae Whole-Cell Extracts.- 26 In Vitro Excision Repair Assay in Schizosaccharomyces pombe.- 27 Nucleotide Excision Repair Assay in Drosophila melanogaster Using Established Cell Lines.- 28 Nucleotide Excision Repair in Nuclear Extracts from Xenopus Oocytes.- 29 Assay for Nucleotide Excision Repair Protein Activity Using Fractionated Cell Extracts and UV-Damaged Plasmid DNA.- 30 Dual-Incision Assays for Nucleotide Excision Repair Using DNA with a Lesion at a Specific Site.- 31 In Vitro Chemiluminescence Assay to Measure Excision Repair in Cell Extracts.- III. DNA Strand Breakage and Repair.- 32 Physical Monitoring of HO-Induced Homologous Recombination.- 33 Use of P Element Transposons to Study DNA Double-Strand Break Repair in Drosophila melanogaster.- 34 Analyzing Double-Strand Repair Events in Drosophila.- 35 Expression of I-Sce I in Drosophila to Induce DNA Double-Strand Breaks.- 36 Use of I-Sce I to Induce DNA Double-Strand Breaks in Nicotiana.- 37 Chromosomal Double-Strand Breaks Introduced in Mammalian Cells by Expression of I-Sce I Endonuclease.- 38 Induction of DNA Double-Strand Breaks by Electroporation of Restriction Enzymes into Mammalian Cells.- 39 In Vitro Rejoining of Double-Strand Breaks in Genomic DNA.- 40 Extrachromosomal Assay for DNA Double-Strand Break Repair.- 41 Use of Gene Targeting to Study Recombination in Mammalian DNA Repair Mutants.- 42 Measurement of Low-Frequency DNA Breaks Using Nucleoid Flow Cytometry.- IV. DNA Damage Tolerance Mechanisms and Regulatory Responses.- 43 Live Analysis of the Division Cycles in X-Irradiated Drosophila Embryos.- 44 Inhibition of DNA Synthesis by Ionizing Radiation.- 45 Analysis of Inhibition of DNA Replication in Irradiated Cells Using the SV40-Based In Vitro Assay of DNA Replication.- 46 Assays of Bypass Replication of Genotoxic Lesions in Mammalian Disease and Mutant Cell-Free Extracts.- 47 Detection of Chromatin-Bound PCNA in Cultured Cells Following Exposure to DNA-Damaging Agents.- 48 Induction of p53 Protein as a Marker for Ionizing Radiation Exposure In Vivo.- 49 Activation of p53 Protein Function in Response to Cellular Irradiation.- 50 Selective Extraction of Fragmented DNA from Apoptotic Cells for Analysis by Gel Electrophoresis and Identification of Apoptotic Cells by Flow Cytometry.- 51 Detection of DNA Strand Breakage in the Analysis of Apoptosis and Cell Proliferation by Flow and Laser Scanning Cytometry.- 52 Immunoassay for Single-Stranded DNA in Apoptotic Cells.