E.coli: Probiotic for Piglet Diarrhoea
Escherichia Coli K88+ post Weaning Diarrhoea
(Sprache: Englisch)
Aim of this study was to select environmental E. coli isolates that produced colicins against the swine pathogen E. coli K88+. In initial evaluation using a modified plate method with 18 colicinogenic E. coli constructs, colicins E3, E4, E5, E9, Ia, K and N...
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Aim of this study was to select environmental E. coli isolates that produced colicins against the swine pathogen E. coli K88+. In initial evaluation using a modified plate method with 18 colicinogenic E. coli constructs, colicins E3, E4, E5, E9, Ia, K and N were found to possess inhibitory activity against 12 ETEC K88+ strains. A total of 463environmental isolates from cattle rumen, cattle feces, pig feces and hog manure-amendedsoil were screened for colicin production by a modified plate test. Further,colicinogenic isolates were screened for five toxin genes LT, STa, STb, VT1 and VT2 as well as K88 (F4) fimbriae using PCR reactions. Fourteen non-pathogenic isolates weresubjected to characterization of colicin genes by PCR using 9 new primer sequences, antibiotic susceptibilities and substrate utilization. Two potential probiotic strains of E. coli, UM-2 and UM-7 which produced colicins that could utilize potato starch and inulin were selected for in-vitro competition with E. coli K88+ strain 2-12. In vitrocompetition between the synbiotics and E. coli K88+ revealed inhibition of E. coli K88+. Animal testing of these strains is currently being undertaken.
Autoren-Porträt von Amit Setia
Setia, AmitDr. Amit Setia is a veterinarian by profession from India. In April, 2007 Completed masters project under supervision of Dr D O Krause at University of Manitoba, Canada. Since Nov. 2007 working as research Fellow on project titled Effect of forced litter consumption in broiler performance at University of New England, Australia.
Bibliographische Angaben
- Autor: Amit Setia
- 2008, 116 Seiten, Maße: 15 x 22 cm, Kartoniert (TB), Englisch
- Verlag: VDM Verlag Dr. Müller e.K.
- ISBN-10: 3639026276
- ISBN-13: 9783639026276
Sprache:
Englisch
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