Forensic DNA Typing Protocols

Collection of Samples for DNA Analysis.- Body Fluid Identification Using mRNA Profiling.- mRNA Profiling for Vaginal Fluid and Menstrual Blood Identification.- Preservation of and DNA Extraction from Muscle Tissue.- DNA Extraction: Organic and Solid-Phase.- Extraction of DNA from Skeletal Remains.- Extraction of DNA from Human Skeletal Material.- The Development and Use of Internal Amplification Controls (IACs) with DNA Profiling Kits for Forensic DNA Analysis.- NucleoSpin ®XS Columns for DNA Concentration and Clean-Up.- Purification of PCR Products to Improve STR Profiles.- Analysis of 30 Biallelic INDEL Markers Using the Investigator DIPplex ® Kit.- Analysis of Mitochondrial Control Region Using Sanger Sequencing.- Whole Human Mitochondrial DNA Sequencing.- In-Solution Hybridization for the Targeted Enrichment of the Whole Mitochondrial Genome.- Enhanced DNA Profiling of the Semen Donor in Late Reported Sexual Assaults: Use of Y-Chromosome Targeted Pre-Amplification and Next Generation Y-STR Amplification Systems.- Analysis of Rapidly Mutating Y Chromosome Short Tandem Repeats (RM Y STRs).- A Practical Guide to the HIrisPlex System: Simultaneous Prediction of Eye and Hair Color from DNA.- Inference of Ancestry in Forensic Analysis I: Autosomal Ancestry - Informative Marker Sets.- Inference of Ancestry in Forensic Analysis II: Analysis of Genetic Data.- Species Determination: The Role and Use of the Cytochrome b Gene.

"This is an instructional book that raises awareness of the technology available in genetic forensic sciences. ... The book is written in a way that is easy to understand for advanced professionals as well as beginners. Anyone wanting to understand the technology involved in forensic DNA analysis would benefit from this unique book. ... The value of this book lies in the simple and logical way it presents complex information in forensic medicine." (Luis F. Escobar, Doody's Book Reviews, November, 2016)