Germ Cell Protocols

Cryopreservation of Semen of the Salmonidae with Special Reference to Large-Scale Fertilization.- Fertilization and Sperm Chemotaxis in Ascidians.- Assays for Vertebrate Sperm Chemotaxis.- Stallion Spermatozoa Viability.- Visualization of Sperm Accessory Structures in the Mammalian Spermatids, Spermatozoa, and Zygotes by Immunofluorescence, Confocal, and Immunoelectron Microscopy.- Localization of Zona Pellucida Receptors on Live Sperm by Fluorophore-Conjugated Solubilized Zona Pellucida Proteins.- Detection of Human Papillomavirus DNA in Sperm Using Polymerase Chain Reaction.- Spermatogonial Stem Cells.- Molecular and Cellular Approaches for Establishment of an In Vitro Spermatogenesis System.- Acrosin Activity Assay for the Evaluation of Mammalian Sperm Acrosome Reaction.- Isolation of Sea Urchin Sperm Plasma Membranes.- Detection of Centrosome Structure in Fertilized and Artificially Activated Sea Urchin Eggs Using Immunofluorescence Microscopy and Isolation of Centrosomes Followed by Structural Characterization with Field Emission Scanning Electron Microscopy.- Microscopic Techniques for Studying Sperm-Oocyte Interaction During Fertilization and Early Embryonic Development.- Immunocytochemical Staining of the Metaphase II Spindle in Mammalian Oocytes.- Spindle Imaging in Living Mammalian Oocytes Using the Liquid-Crystal Polarized Light Microscope and Its Practical Use.- In Vitro Maturation and Fertilization of Pig Oocytes.- In Vitro Maturation and In Vitro Fertilization of Human Oocytes.- In Vitro Maturation and Fertilization of Canine Oocytes.- Detection and Measurement of Membrane-Bound Protein Tyrosine Kinases in the Zebrafish Egg.- Single-Cell Method for Estimation of Protein Tyrosine Kinases in the Zebrafish Egg.- Activity of MAPK/p90rsk During Fertilization inMice, Rats, and Pigs.