High-Frequency In vitro Plant Regeneration of Black gram and Grass pea

Vigna mungo L. (Black gram) and Lathyrus sativus L. (Grass pea) are leguminous crop plants cultivated extensively in various parts of the world due to their qualities of being a very cheap source of diet protein. They also considered as fodder plants along with their excellent N2-fixation properties and high resistance to different agricultural stresses. The high-frequency micropropagation protocol of Black gram and Grass pea was optimized. The frequency of callus formation was highest for Black gram on Murashige and Skoog (MS) medium supplemented with high concentrations of 2,4-D using young and green leaf. Callus obtained from hypocotyl segments of Black gram at high concentration of BAP are highly amenable to produce multiple shoots. Plantlets were regenerated from embryogenic callus while BAP and NAA were used. Direct shoots through organogenesis were induced from cotyledonary nodes of grass pea on MS medium with Gamborg B5 vitamin containing 6-benzylaminopurine (BAP), Thidiazuron (TDZ) and -naphthalene acetic acid (NAA). Regenerated plantlets and multiple shoots were rooted most effectively in half strength MS medium containing different concentration of NAA and IBA.