High Performance Liquid Chromatography
(Sprache: Englisch)
Modern liquid column chromatography (LC) has developed rapidly since 1969 to become a standard method of separation. If the statisticians are to be believed, the recent growth of LC has been the most specta cular development in analytical chemistry and has...
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Klappentext zu „High Performance Liquid Chromatography “
Modern liquid column chromatography (LC) has developed rapidly since 1969 to become a standard method of separation. If the statisticians are to be believed, the recent growth of LC has been the most specta cular development in analytical chemistry and has not yet abated be cause its vast potential for application remains to be fully exploit ed. Significant factors contributing to this continued rise are the simplicity and low cost of the required basic equipment and the rela tive ease of acquiring and interpreting the data. Unfortunately, in LC, as so often in the field of analytical chemistry, the available commercial instruments are frequently far more complicated - and consequently far more expensive - than is nec essary for routine application. Therein also lies the risk of propa gating a "black box" philosophy that would be particularly detrimen tal to chromatography. Moreover, it appears to have been forgotten, as was done previously with gas chromatography, that inadequate sep aration by a column can be remedied only with great difficulty, if at all, by electronic means. Also, whether the capillary columns recent ly advocated with great enthusiasm for LC will fulfill the expecta tions of their proponents is highly questionable unless someone comes up with some new and revolutionary ideas.
Inhaltsverzeichnis zu „High Performance Liquid Chromatography “
I. Chromatographic Processes.- References Chapter I.- II. Fundamentals of Chromatography.- A. Retention.- B. Linear Flow Rate, Porosity, Permeability.- C. Band Broadening.- D. Resolution.- E. Dependence of Band Broadening on Flow Rate.- F. Band Broadening and Particle Size.- G. Extra-Column Band Broadening.- H. Optimum Analysis Conditions and Analysis Time.- I. Selection of a Suitable Column.- References Chapter II.- III. Equipment for HPLC.- A. Solvent Reservoir - Degassing of the Eluent.- B. Pumps.- C. Damping of the Pulsations.- D. Sample Introduction.- E. The Column.- F. Thermostating.- G. Measurement of the Flow Rate.- H. Fraction Collectors.- I. Recorders.- J. Instrumentation for Gradient Elution.- K. Safety Measures.- References Chapter III.- IV. Detectors.- A. UV Detectors.- B. Differential Refractometer.- C. Microadsorption Detector.- D. Transport Detector (Flame Ionization Detector).- E. Fluorescence Detector.- F. Other Detectors.- G. Comparison of the Important Detectors.- H. Reaction Detectors.- References Chapter IV.- V. Stationary Phases.- A. Packing Materials for Adsorption and Partition Chromatography.- B. Chemically Modified Supports.- C. Ion Exchangers.- D. Stationary Phases for Exclusion Chromatography.- References Chapter V.- VI. Adsorption Chromatography.- I. Polar Stationary Phases.- II. Nonpolar Stationary Phases.- III. The General Elution Problem.- IV. Applications of Adsorption Chromatography.- References Chapter VI.- VII. Partition Chromatography.- A. Introduction.- B. Supports and Liquid Phases.- C. Properties of the Column.- D. Applications.- E. Ion-pair Chromatography.- References Chapter VII.- VIII. Ion-Exchange Chromatography.- A. Principle.- B. Ion Exchange Materials.- C. Characterization of the Ion Exchangers.- D. Optimizing aSeparation.- E. Applications.- References Chapter VIII.- IX. Exclusion Chromatography. Gel Permeation Chromatography.- A. Introduction.- B. Basics of Exclusion Chromatography.- C. Stationary Phases for Exclusion
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Chromatography.- D. Applications of Exclusion Chromatography.- References Chapter IX.- X. Selection of the Separation System.- References Chapter X.- XI. Special Techniques.- A. Preparative Chromatography.- B. Qualitative Analysis.- C. Quantitative Analysis.- D. Trace Analysis.- References Chapter XI.- XII. Purification of Solvents.- References Chapter XII.
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Bibliographische Angaben
- Autor: Heinz Engelhardt
- 2012, Softcover reprint of the original 1st ed. 1979, XII, 254 Seiten, 73 Abbildungen, Maße: 24,4 cm, Kartoniert (TB), Englisch
- Übersetzer: G. Gutnikov
- Verlag: Springer, Berlin
- ISBN-10: 3642670660
- ISBN-13: 9783642670664
Sprache:
Englisch
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