HPTLC based Phyto-chemical Fingerprinting of Certain Indian Plants

Plants produce several secondary metabolites with varying characteristics such as alkaloids, terpenoids, flavanoids, steroids, saponins, glycosides, tannins etc. The book includes HPTLC based Phyto-chemical fingerprinting monographs of extracts of thirty seven Indian plants and four essential oils with detailed extraction methods and HPTLC sample preparation. The study serves a base work for further analysis of individual compound and searching their unnoticed bioactivity. Nowadays HPTLC is a routine analytical technique, facilitated with very easy sample preparation and multiple sample analysis on a same plate and solvent system, thereby saving a time. Also simultaneous chromatographic analysis of samples and standards on the same plate under identical conditions gives results with better accuracy and precision. Another advantage of HPTLC is that, the chromatogram can be scanned at broad range of wavelengths and recorded in computer as a digital profile and used as a reference and further comparative analysis. Thus HPTLC is a modern standardization technique having a large applicability in the field of plant material analysis, stability tests of extracts and finished products.