Laboratory Protocols in Fungal Biology

Detection and Quantification of Aspergillus fumigatus DNA in Clinical Samples of Neutropenic Patients.- Application of Polymerase Chain Reaction (PCR) and PCR Based Methods Targeting Internal Transcribed Spacer (ITS) for Detection and Species Level Identification of Fungi.- Real-Time PCR Assay in Fungi.- Quantitative Sampling Methods for the Analysis of Fungi: Air Sampling.- Transformation of Filamentous Fungi in Microtiter Plate.- Molecular Fingerprinting of Fungal Communities in Soil.- Development of Microsatellite Markers from Fungal DNA Based on Shotgun Pyrosequencing.- Multiplex and Quantifiable Detection of Infectious Fungi Using Padlock Probes, General qPCR and Suspension Microarray Readout.- Rapid Deletion Plasmid Construction Methods for Protoplast and Agrobacterium-based Fungal Transformation Systems.- Improved Transformation Method for Alternaria brassicicola and its Applications.- Methods for High Quality DNA Extraction from Fungi.- Production of Recombinant Proteins from Pichia pastoris: Interfacing Fermentation and Immobilized Metal Ion Affinity Chromatography.- Development of a Real-Time Quantitative PCR Assay for the Assessment of Uncultured Zoosporic Fungi.- Nucleic and Protein Extraction Methods for Fungal Exopolysaccharide Producers.- Directed Evolution of A Fungal Xylanase for Improvement of Thermal and Alkaline Stability.- Genome Shuffling Protocol for the Pentose-Fermenting Yeast Scheffersomyces stipitis.- Detection and Identification of Fungal Microbial Volatile Organic Compounds by HS-SPME-GC-MS.- Transformation Methods for Slow Growing Fungi.- Enzymatic Saccharification of lignocellulosic biomass.- Protoplast Fusion Techniques in Fungi.- Large Scale Production of Lignocellulolytic Enzymes in Thermophilic Fungi.- Panfungal PCR Method for Detection of Aflatoxigenic Molds.- Protocols for the Quantification of dsDNA and Its Fragmentation Status in Fungi.- Rapid Identification and Detection of Pathogenic Fungi by Padlock Probes.- Drug-Induced Permeabilization in Fungi.- Extraction and Characterization of Taxol: An Anticancer Drug from an Endophytic And Pathogenic Fungi.- Identification of Mycotoxigenic Fungi using an Oligonucleotide Microarray.- DNA Microarray-Based Detection and Identification of Fungal Specimens.- Bioinformatic Protocols and the Knowledge-Base for Secretomes in Fungi.- High-Throughput Functional Annotation and Data Mining of Fungal Genomes to Identify Therapeutic Targets.- Application of Support Vector Machines in Fungal Genome and Proteome Annotation.- Bioinformatics Tools for the Multilocus Phylogenetic Analysis of Fungi.

Ireland. He has three years of research experience in microbial biotechnology. Dr. Anthonia O'Donovan is a Postdoctoral Research Scientist with NUIG, Ireland. She obtained her PhD in Biochemistry from the National University of Ireland Galway, Ireland. She has seven years of research experience in microbial enzymes and biofuels technology.