Lipoprotein Protocols

Part I: dna and rna Based Methodology.PCR Methodology Applied to Genetic Studies of Lipoprotein Metabolism and Atherosclerosis,Shelley A. Cole and James E. Hixson. Separation of Small-Size DNA Fragments Using Agarose Gel Electrophoresis,Jose M. Ordovas. Quantification of mRNA by Polymerase Chain Reaction (PCR) Using an Internal Standard and a Nonradioactive Detection Method,William H. Karge, III, Ernst J. Schaefer, and Jose M. Ordovas. Generation of Transgenic Mice, Marten H. Hofker and Marco Breuer. Preparation of Knockout Mice, Jesús Osada and Nobuyo Maeda. Part II.Protein Based Methodology.Fast Ultracentrifugation Methods for the Separation of Plasma Lipoproteins,Jose M. Ordovas. Preparative Isolation of Plasma Lipoproteins Using Fast Protein Liquid Chromatography,Jose M. Ordovas and Doreen Osgood. Separation of Polipoproteins by Polyacrylamide Gel Electrophoresis,Jose M. Ordovas. Isolation and Purification of Serum Lipoprotrein (a) Using Lectin Affinity,Leo J. Seman, Carl de Luca, and Jennifer L. Jenner. Electrophoretic Separation of LDL and HDL Subclasses David L. Rainwater. Measurement of Lipoprotein Particles,Catherine Fievet and Jean-Charles Fruchart. Methodological Approaches for Assessing Lipid and Protein Oxidation and Modification in Plasma and Isolated Lipoproteins,Wolfgang Sattler, Ernst Malle, and Gert M. Kostner. Separation and Quantitation of Phospholipid Classes by HPLC,George M. Patton and Sander J. Robins. Assays of Lecithin Cholesterol Acyltransferase (LCAT), Milada Dobiásová and Jiri J. Frohlich. Determination of the Mass Concentration and the Activity of the Plasma Cholesteryl Ester Transfer Protein (CETP),Laurent Lagrost. In Vitro Measurement of Lipoprotein and Hepatic Lipases,Elisabet Vilella and Jorge Joven. Cellular Assays for Lipoprotein Receptors,Susan Acton and Attilio Rigotti . Measurements of Proteoglycan-Lipoprotein Interaction by Gel Mobility Shift Assay,Eva Hurt-Camejo, Germán Camejo, and PeterSartipy.

This text makes a good selection with admirable breadth and a high general quality of method description. - Clin Chem Lab Med The book presents the state-of-art of molecular biology and protein techniques specific for lipoprotein research...The book may be especially recommended for students who start working on lipoproteins, but may also be generally helpful for molecular biologists... - Nahrung Each chapter contains, besides a description of the principle of the methods, highly instructive protocols and lists of the technical requirements and reagents. Notes with additional information, e.g. special experimental conditions or safety and technical advises as well as numerous references are given. The book may be especially recommended for students who start working on lipoproteins, but may also be generally helpful for molecular biologists. - Nahrung