Molecular and Modern Studies on Potato leaf roll virus (PLRV)
Viral & Tissue Culture Study
(Sprache: Englisch)
This investigation was carried out to achieve the following: 1. Conducting some molecular studies on PLRV such as : - Extraction of RNA - Reverse transcriptiom reaction - PCR - Purification of PCR product - Cloning of PCR product - Large scale plasmid...
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This investigation was carried out to achieve the following: 1. Conducting some molecular studies on PLRV such as : - Extraction of RNA - Reverse transcriptiom reaction - PCR - Purification of PCR product - Cloning of PCR product - Large scale plasmid preparation(Maxi-prep) - Subcloning - Large scale plasmid preparation(Maxi-prep) 2. Using of modern serological methods for rapid detection of PLRV such as: - ELISA - DBIA - TBIA 3. Recognize the intracellular changes induced by the PLRV using Electron Microscopy 4. Production of PLRV-free potato plants.
Bibliographische Angaben
- Autor: Emad Fathy Mohamed Dwidar
- 2014, 152 Seiten, Maße: 22 cm, Kartoniert (TB), Englisch
- Verlag: LAP Lambert Academic Publishing
- ISBN-10: 3659553441
- ISBN-13: 9783659553448
Sprache:
Englisch
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