Nanotechnology for Nucleic Acid Delivery
Methods and Protocols
(Sprache: Englisch)
This detailed second edition volume expands upon the prior edition by addressing newly emerged technologies as well as improved existing protocols in nucleic acid delivery via nanotechnology. The book addresses topics ranging from chemical synthesis of...
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Klappentext zu „Nanotechnology for Nucleic Acid Delivery “
This detailed second edition volume expands upon the prior edition by addressing newly emerged technologies as well as improved existing protocols in nucleic acid delivery via nanotechnology. The book addresses topics ranging from chemical synthesis of macromolecules and bioconjugates, novel and established nanoformulations, characterization of these nanoformulations for biophysical, biological and toxicological aspects, and also protocols dealing with application and imaging of such carrier systems in vivo. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective chapters, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Nanotechnology for Nucleic Acid Delivery: Methods and Protocols, Second Edition serves as an ideal guide to researchers seeking to use this vital area of study in their own lab work or to progress knowledge within the field itself.
Inhaltsverzeichnis zu „Nanotechnology for Nucleic Acid Delivery “
1. Sequence-Defined Cationic Lipo-Oligomers Containing Unsaturated Fatty Acids for TransfectionSören Reinhard and Ernst Wagner
2. Synthesis of Bioreducible Polycations with Controlled Topologies
Ye-Zi You, Jun-Jie Yan, Fei Yu, Zhi-Qiang Yu, and David Oupický
3. Histidine-Rich Cationic Cell Penetrating Peptides for Plasmid DNA and siRNA Delivery
Antoine Kichler, A. James Mason, Arnaud Marquette, and Burkhard Bechinger
4. Covalent Fluorophore Labeling of Oligonucleotides and Generation of Other Oligonucleotide Bioconjugates
Cornelia Lorenzer and Johannes Winkler
5. Synthetic Oligonucleotides in SPECT/CT In Vivo Imaging: Chemical Modifications, In111 Complex Formation, Incorporation into Drug Delivery Systems
Maxim Antopolsky
6. Synthesis of Polyethylenimine-Based Nanocarriers for Systemic Tumor Targeting of Nucleic Acids
Wolfgang Rödl, Alexander Taschauer, David Schaffert, Ernst Wagner, and Manfred Ogris
7. Cationic Photopolymerized Polydiacetylenic (PDA) Micelles for siRNA Delivery
Manon Ripoll, Patrick Neuberg, Jean-Serge Remy, and Antoine Kichler
8. Lipids for Nucleic Acid Delivery: Cationic or Neutral Lipoplexes, Synthesis, and Particle Formation
Michel Bessodes, Helene Dhotel, and Nathalie Mignet
9. Preparation, Characterization, and In Vitro Evaluation of Lipidoid-Polymer Hybrid Nanoparticles for siRNA Delivery to the Cytosol
Kaushik Thanki, Xianghui Zeng, and Camilla Foged
10. Layer-By-Layer Assembled Nanoparticles for siRNA Delivery
Michaela Guter and Miriam Breunig
11. Layer-By-Layer Film Engineering for Sequential Gene Delivery
Lingxiao Xie, Yi Zou, Sean Carroll, Maria Muniz, and Guangzhao Mao
12. Surface- and Hydrogel-Mediated Delivery of Nucleic Acid Nanoparticles
Angela K. Pannier, Tyler Kozisek, and Tatiana Segura
13. In Situ AFM
... mehr
Analysis Investigating Disassembly of DNA Nanoparticles and Nano-Films
Yi Zou, Lei Wan, Jenifer Blacklock, Lingxiao Xie, Sean Carroll, David Oupicky, and Guangzhao Mao
14. Lyophilization of Synthetic Gene Carriers
Julia Christina Kasper, Sarah Hedtrich, and Wolfgang Friess
15. Firefly Luciferase-Based Reporter Gene Assay for Investigating Nanoparticle-Mediated Nucleic Acid Delivery
Katharina Müller, Manfred Ogris, and Haider Sami
16. Enhancing Nucleic Acid Delivery with Ultrasound and Microbubbles Heleen Dewitte, Silke Roovers, Stefaan C. De Smedt, and Ine Lentacker
17. Magnetic and Acoustically Active Microbubbles Loaded with Nucleic Acids for Gene Delivery
Dialechti Vlaskou, Olga Mykhaylyk, and Christian Plank
18. Lactate Dehydrogenase Assay for Assessment of Polycation Cytotoxicity
Ladan Parhamifar, Helene Andersen, and S. Moein Moghimi
19. Combined Fluorimetric Caspase 3/7 Assay and Bradford Protein Determination for Assessment of Polycation-Mediated Cytotoxicity
Anna K. Larsen, Arnaldur Hall, Henrik Lundsgart, and S. Moein Moghimi
20. Determination of Polycation-Mediated Perturbation of Mitochondrial Respiration in Intact Cells by High-Resolution Respirometry (Oxygraph-2k, OROBOROS)
Arnaldur Hall and S. Moein Moghimi
21. Evaluating the Regulation of Cytokine Levels after siRNA Treatment in Antigen-Specific Target Cell Populations via Intracellular Staining
Rima Kandil, Daniel Feldmann, Yuran Xie, and Olivia M. Merkel
22. Anti-PEG IgM Production via a PEGylated Nano-Carrier System for Nucleic Acid Delivery
Amr S. Abu Lila and Tatsuhiro Ishida
23. Near-Infrared Optical Imaging of Nucleic Acid Nanocarriers In Vivo
Claire Rome, Julien Gravier, Marie Morille, Gilles Divita, Anne-Laure Bolcato-Bellemin, Véronique Josserand, and Jean-Luc Coll
24. Flow Cytometry-Based Cell Type-Specific Assessment of Target Regulation by Pulmonary siRNA Delivery
Olivia M. Merkel, Leigh M. Marsh, Holger Garn, and Thomas Kissel
25. Microbubbles for Nucleic Acid Delivery in Liver Using Mild Sonoporation
Nathalie Mignet, Corinne Marie, Anthony Delalande, Simona Manta, Michel-Francis Bureau, Gilles Renault, Daniel Scherman, and Chantal Pichon
26. Lipopeptide Delivery of siRNA to the Central Nervous System
Mark D. Zabel, Luke Mollnow, and Heather Bender
Yi Zou, Lei Wan, Jenifer Blacklock, Lingxiao Xie, Sean Carroll, David Oupicky, and Guangzhao Mao
14. Lyophilization of Synthetic Gene Carriers
Julia Christina Kasper, Sarah Hedtrich, and Wolfgang Friess
15. Firefly Luciferase-Based Reporter Gene Assay for Investigating Nanoparticle-Mediated Nucleic Acid Delivery
Katharina Müller, Manfred Ogris, and Haider Sami
16. Enhancing Nucleic Acid Delivery with Ultrasound and Microbubbles Heleen Dewitte, Silke Roovers, Stefaan C. De Smedt, and Ine Lentacker
17. Magnetic and Acoustically Active Microbubbles Loaded with Nucleic Acids for Gene Delivery
Dialechti Vlaskou, Olga Mykhaylyk, and Christian Plank
18. Lactate Dehydrogenase Assay for Assessment of Polycation Cytotoxicity
Ladan Parhamifar, Helene Andersen, and S. Moein Moghimi
19. Combined Fluorimetric Caspase 3/7 Assay and Bradford Protein Determination for Assessment of Polycation-Mediated Cytotoxicity
Anna K. Larsen, Arnaldur Hall, Henrik Lundsgart, and S. Moein Moghimi
20. Determination of Polycation-Mediated Perturbation of Mitochondrial Respiration in Intact Cells by High-Resolution Respirometry (Oxygraph-2k, OROBOROS)
Arnaldur Hall and S. Moein Moghimi
21. Evaluating the Regulation of Cytokine Levels after siRNA Treatment in Antigen-Specific Target Cell Populations via Intracellular Staining
Rima Kandil, Daniel Feldmann, Yuran Xie, and Olivia M. Merkel
22. Anti-PEG IgM Production via a PEGylated Nano-Carrier System for Nucleic Acid Delivery
Amr S. Abu Lila and Tatsuhiro Ishida
23. Near-Infrared Optical Imaging of Nucleic Acid Nanocarriers In Vivo
Claire Rome, Julien Gravier, Marie Morille, Gilles Divita, Anne-Laure Bolcato-Bellemin, Véronique Josserand, and Jean-Luc Coll
24. Flow Cytometry-Based Cell Type-Specific Assessment of Target Regulation by Pulmonary siRNA Delivery
Olivia M. Merkel, Leigh M. Marsh, Holger Garn, and Thomas Kissel
25. Microbubbles for Nucleic Acid Delivery in Liver Using Mild Sonoporation
Nathalie Mignet, Corinne Marie, Anthony Delalande, Simona Manta, Michel-Francis Bureau, Gilles Renault, Daniel Scherman, and Chantal Pichon
26. Lipopeptide Delivery of siRNA to the Central Nervous System
Mark D. Zabel, Luke Mollnow, and Heather Bender
... weniger
Bibliographische Angaben
- 2019, 2. Aufl., 406 Seiten, 71 farbige Abbildungen, Maße: 18,3 x 26 cm, Gebunden, Englisch
- Herausgegeben: Manfred Ogris, Haider Sami
- Verlag: Springer, Berlin
- ISBN-10: 1493990918
- ISBN-13: 9781493990917
- Erscheinungsdatum: 06.03.2019
Sprache:
Englisch
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