Phospho-Proteomics
Methods and Protocols
(Sprache: Englisch)
This second edition provides up-to-date protocols focusing on techniques for the specific enrichment of phosphopeptides and phosphoproteins. Phosphoproteomics Methods and Protocols, Second Edition guides readers through different labeling strategies for...
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Klappentext zu „Phospho-Proteomics “
This second edition provides up-to-date protocols focusing on techniques for the specific enrichment of phosphopeptides and phosphoproteins. Phosphoproteomics Methods and Protocols, Second Edition guides readers through different labeling strategies for quantitative phosphoproteomics; high-throughput mass spectrometry-based phosphoproteome analyses and phospho flow cytometry; and bioinformatics strategies for phosphoproteomics data analysis and integration. Additional protocols concentrate on the identification of kinase-substrate relationships by both high- and low-throughput approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Phosphoproteomics Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.
Inhaltsverzeichnis zu „Phospho-Proteomics “
Part I: Techniques for Tagging Phospho-Moieties1. Thiol-ene Enabled Detection of Thiophosphorylation as a Labeling Strategy for Phosphoproteins
Kaelyn E. Wilke and Erin E. Carlson
2. Phosphopeptide Detection with Biotin-Labeled Phos-tag
Emiko Kinoshita-Kikuta, Eiji Kinoshita and Tohru Koike
3. Phosphopeptide Enrichment by Covalent Chromatography after Solid Phase Derivatization of Protein Digests on Reversed Phase Supports
Heinz Nika,Ruth Hogue Angeletti, and David H. Hawke
Part II: Labeling Techniques for Quantitative Phosphoproteomics
4. Peptide Labeling Using Isobaric Tagging Reagents for Quantitative Phosphoproteomics
Lei Cheng, Trairak Pisitkun, Mark A. Knepper, and Jason D. Hoffert
5. Identification of Direct Kinase Substrates Using Analogue-Sensitive Alleles
Daniel A. Rothenberg, Elizabeth A. Gordon, Forest M. White, and Sebastian Lourido
6. Quantitative Analysis of Tissue Samples by Combining iTRAQ Isobaric Labeling with Selected/Multiple Reaction Monitoring (SRM/MRM)
Ryohei Narumi and Takeshi Tomonaga
Part III: Phosphopeptide Enrichment Strategies
7. Enrichment Strategies in Phosphoproteomics
Alexander Leitner
8. Phosphopeptide Enrichment by Immobilized Metal Affinity Chromatography
Tine E. Thingholmand Martin R. Larsen
9. The use of Titanium Dioxide for Selective Enrichment of Phosphorylated Peptides
Tine E. Thingholm and Martin R. Larsen
10. Sequential Elution from IMAC (SIMAC) - An Efficient Method for Enrichment and Separation of Mono- and Multi-Phosphorylated Peptides
Tine E. Thingholm and Martin R. Larsen
11. Improving the Phosphoproteome Coverage for Limited Sample Amounts Using TiO2-SIMAC-HILIC (TiSH) Phosphopeptide Enrichment and Fractionation
Kasper Engholm-Keller and Martin R. Larsen
12. Offline High pH Reversed-Phase Peptide Fractionation for Deep Phosphoproteome Coverage
Tanveer S Batth, Jesper V Olsen
13. Phosphopeptide Enrichment Using Various Magnetic Nanocomposites - An Overview
Íris L. Batalha and Ana Cecília
... mehr
A. Roque
Part IV: Phosphoprotein Analysis by Mass Spectrometry
14. Two Dimensional Gel Electrophoresis-Based Plant Phosphoproteomics
Chao Hanand Pingfang Yang
15. Variable Digestion Strategies for Phosphoproteomics Analysis
Humberto Gonczarowska-Jorge, Margherita Dell'Aica, Clarissa Dickhut, and René P. Zahedi
16. On-line LC-FAIMS-MS/MS for the Analysis of Phosphorylation in Proteins
Hongyan Zhao, Andrew J. Creese, and Helen J. Cooper
17. Simple and Reproducible Sample Preparation for Single Shot Phosphoproteomics With High Sensitivity
Rosa R. Jersie-Christensen, Abida Sultan, and Jesper V. Olsen
Part V: High-throughput Studies of the Phosphoproteome
18. Identification of Direct Kinase Substrates via Kinase Assay Linked Phosphoproteomics
Liang Xue, Justine V. Arrington, and W. Andy Tao
19. Phosphoprotein Detection by High-Throughput Flow Cytometry
Johannes Landskronand Kjetil Taskén
Part VI: Bioinformatics Resources for Phosphoproteomics Analyses
20. Resources for Assignment of Phosphorylation Sites on Peptides and Proteins
Vaishnavi Ravikumar,Boris Macek, and Ivan Mijakovic
21. From Phosphosites to Kinases
Stephanie Munk, Jan C. Refsgaard, and Jesper V. Olsen, and Lars J. Jensen
22. Search Databases and Statistics - Pitfalls and Best Practices in Phosphoproteomics
Jan C. Refsgaard, Stephanie Munk, and Lars J. Jensen
23. Systems Analysis for Interpretation of Phosphoproteomics Data
Stephanie Munk, Jan C. Refsgaard, and Jesper V. Olsen
Part IV: Phosphoprotein Analysis by Mass Spectrometry
14. Two Dimensional Gel Electrophoresis-Based Plant Phosphoproteomics
Chao Hanand Pingfang Yang
15. Variable Digestion Strategies for Phosphoproteomics Analysis
Humberto Gonczarowska-Jorge, Margherita Dell'Aica, Clarissa Dickhut, and René P. Zahedi
16. On-line LC-FAIMS-MS/MS for the Analysis of Phosphorylation in Proteins
Hongyan Zhao, Andrew J. Creese, and Helen J. Cooper
17. Simple and Reproducible Sample Preparation for Single Shot Phosphoproteomics With High Sensitivity
Rosa R. Jersie-Christensen, Abida Sultan, and Jesper V. Olsen
Part V: High-throughput Studies of the Phosphoproteome
18. Identification of Direct Kinase Substrates via Kinase Assay Linked Phosphoproteomics
Liang Xue, Justine V. Arrington, and W. Andy Tao
19. Phosphoprotein Detection by High-Throughput Flow Cytometry
Johannes Landskronand Kjetil Taskén
Part VI: Bioinformatics Resources for Phosphoproteomics Analyses
20. Resources for Assignment of Phosphorylation Sites on Peptides and Proteins
Vaishnavi Ravikumar,Boris Macek, and Ivan Mijakovic
21. From Phosphosites to Kinases
Stephanie Munk, Jan C. Refsgaard, and Jesper V. Olsen, and Lars J. Jensen
22. Search Databases and Statistics - Pitfalls and Best Practices in Phosphoproteomics
Jan C. Refsgaard, Stephanie Munk, and Lars J. Jensen
23. Systems Analysis for Interpretation of Phosphoproteomics Data
Stephanie Munk, Jan C. Refsgaard, and Jesper V. Olsen
... weniger
Bibliographische Angaben
- 2015, 2. Aufl., XIII, 364 Seiten, Maße: 18,3 x 26 cm, Gebunden, Englisch
- Herausgegeben: Louise von Stechow
- Verlag: Springer, Berlin
- ISBN-10: 1493930486
- ISBN-13: 9781493930487
- Erscheinungsdatum: 20.11.2015
Sprache:
Englisch
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