Phytochemicals as Glucosidase and Amylase Inhibitors

In present study, different plant samples were extracted in different solvents based on their polarity. The obtained extracts which were later on screened for -amylase inhibitory potential. The extracts with -amylase inhibitory potential were afterwards examined for TLC profiling which revealed different band patterns of phytoconstituents. The extracts screened after TLC-Autography, were then tested out for presence of phytochemicals like proteins, carbohydrates, phenols and tannins, flavonoids, saponins, glycosides, steroids, terpenoids, alkaloids, phlobatannins, fixed oils and fatty acids. Subsequently, pos extracts were characterized by HRLC-MS which revealed presence of about 275 compounds. The obtained compounds were then screened for Lipinski rule of five which revealed 116 compounds to satisfy all the five rules. In silico analysis of all 116 compounds for -amylase and -glucosidase inhibition showed more or less inhibitory potential, where we found 15 compounds that showed binding energies less than -10 kcal/mol. Current work is focus on Phytochemicals and their interaction with glucosidase and amylase inhibitors as potential anti-diabetic agents.