Placenta and Trophoblast
Methods and Protocols
(Sprache: Englisch)
A collection of cutting-edge laboratory techniques for the study of trophoblast and placental biology. The techniques presented range from experimental animal models, to animal and human placental organ and cell culture systems, to morphological,...
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Klappentext zu „Placenta and Trophoblast “
A collection of cutting-edge laboratory techniques for the study of trophoblast and placental biology. The techniques presented range from experimental animal models, to animal and human placental organ and cell culture systems, to morphological, biochemical, and molecular strategies for assessing trophoblast/placental growth, differentiation and function. Volume 1 provides readily reproducible protocols for studying embryo-uterine implantation, trophoblast cell development, and the organization and molecular characterization of the placenta. Highlights include strategies for the isolation and culture of trophoblast cells from primates, ruminants, and rodents, and precise guidance to the molecular and cellular analysis of the placental phenotype. A companion second volume concentrates on methods for investigating placental function.
A collection of cutting-edge laboratory techniques for the study of trophoblast and placental biology. The techniques presented range from experimental animal models, to animal and human placental organ and cell culture systems, to morphological, biochemical, and molecular strategies for assessing trophoblast/placental growth, differentiation and function. Volume 1 provides readily reproducible protocols for studying embryo-uterine implantation, trophoblast cell development, and the organization and molecular characterization of the placenta. Highlights include strategies for the isolation and culture of trophoblast cells from primates, ruminants, and rodents, and precise guidance to the molecular and cellular analysis of the placental phenotype. A companion second volume concentrates on methods for investigating placental function.
Inhaltsverzeichnis zu „Placenta and Trophoblast “
Part I. IntroductionPlacenta and Trophoblast: Methods and Protocols: Overview IMichael J. Soares and Joan S. HuntPart II. Methods for Studying Embryo Implantation and Uterine BiologyMethodologies to Study Implantation in MiceKaushik Deb, Jeff Reese, and Bibhash C. PariaBlastocyst CultureD. Randall ArmantIsolation of Hormone Responsive Uterine Stromal Cells: An In Vitro Model for Stromal Cell Proliferation and DifferentiationVirginia RiderRat Decidual Cell CulturesYan Gu and Geula GiboriThe Immortalization of Human Endometrial CellsGraciela Krikun, Gil Mor, and Charles LockwoodSheep Uterine Gland Knockout (UGKO) ModelThomas E. Spencer and C. Allison GrayA Baboon Model for Inducing EndometriosisAsgerally T. FazleabasA Baboon Model for Simulating PregnancyAsgerally T. FazleabasThe Common Marmoset Monkey as a Model for Implantation and Early Pregnancy ResearchAlmuth Einspanier, Kai Lieder, Ralf Einspanier, and Bettina HusenPart III. In Vitro Trophoblast and Placental Model SystemsMouse Trophoblast Stem CellsJennifer Quinn, Tilo Kunath, and Janet RossantConnexins and Trophoblast Cell Lineage DevelopmentMark Kibschull and Elke WinterhagerRcho-1 Trophoblast Stem Cells: A Model System for Studying Trophoblast Cell DifferentiationNamita Sahgal, Lindsey N. Canham, Brent Canham, and Michael J. SoaresBovine Trophoblast Cell Culture Systems: A Technique to Culture Bovine Trophoblast Cells Without Feeder CellsKazuyoshi Hashizume, Arata Shimada, Haruo Nakano, and Toru TakahashiIn Vitro Induction of Trophoblast from Human Embryonic Stem CellsRen-He XuIsolation and Culture of Term Human Trophoblast CellsMargaret G. Petroff, Teresa A. Phillips, Hakhyun Ka, Judith L. Pace, andJoan S. HuntProduction of Human Trophoblast Cell LinesGuy St J. WhitleyCulture and Transfection of Human Choriocarcinoma CellsMichael W. WolfeIn Vitro Methods for Studying Vascularization of the Murine Allantois and Allantoic Union with the ChorionKaren M. DownsPart IV. Phenotypic Analysis of the
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PlacentaPhenotypic Analysis of the Mouse PlacentaDavid R. C. Natale, Maja Starovic, and James C. CrossPhenotypic Analysis of the Rat PlacentaRupasri Ain, Toshihiro Konno, Lindsey N. Canham, and Michael J. SoaresAnalysis of the Structure of the Ruminant Placenta: Methods of Fixation, Embedding, and Antibody Localization at Light and Electron Microscope LevelsF. B. P. WoodingCharacterization of the Bovine Placenta by Cytoskeleton, Integrin Receptors, and Extracellular MatrixChristiane D. PfarrerMolecular Markers for Human Placental InvestigationBerthold HuppertzCorrelative Microscopy of Ultrathin Cryosections in Placental ResearchToshihiro Takizawa and John M. RobinsonVascular Corrosion Casting of the Uteroplacental and Fetoplacental Vasculature in MiceKathie J. Whiteley, Christiane D. Pfarrer, and S. Lee AdamsonAnalysis of Fetal and Maternal Microvasculature in Ruminant Placentomes by Corrosion CastingRudolf Leiser and Christiane D. PfarrerPart V. Molecular Analysis and Gene Transfer TechniquesMicroarray Analysis of Trophoblast CellsVikram Budhraja and Yoel SadovskyGene Expression Microarray Data Analysis of Decidual and Placental Cell DifferentiationSue Kong, Bruce J. Aronow, and Stuart HandwergerAssays to Determine Allelic Usage of Gene Expression in the PlacentaPaul B. VranaAdenoviral-Mediated Gene Delivery to Trophoblast CellsBing Jiang and Carole R. MendelsonIndex
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Bibliographische Angaben
- Autor: Soares
- 2006, 470 Seiten, 145 Abbildungen, Maße: 16,4 x 24 cm, Gebunden, Englisch
- Herausgegeben: Michael J. Soares, Joan S. Hunt
- Verlag: Humana Press
- ISBN-10: 1588294048
- ISBN-13: 9781588294043
- Erscheinungsdatum: 01.11.2005
Sprache:
Englisch
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