Plant Functional Genomics

Carotenoid Extraction from Plant Tissues.- Sample Preparation from Plant Tissue for Gas Chromatography-Mass Spectrometry (GC-MS).- Identification of Volatiles in Tomato Fruit Using Headspace Solid-Phase-Micro-Extraction (HS-SPME) Coupled with Gas Chromatography-Mass Spectrometry (GC-MS).- Determination of Molecular Dimensions of Carbohydrate Polymers (Polysaccharides) by Size Exclusion Chromatography (SEC).- Monosaccharide Analysis after One-Pot Derivatization Followed by Reverse-Phase Liquid Chromatography Separation and UV/Vis Detection.- Atomic Force Microscopy in the Characterization of the Structure of Cell Wall Components.- Studying Plant Specialized Metabolites Using Computational Metabolomics Strategies.- pyPGCF: A Python Software for Phylogenomic Analysis, Species Demarcation, Identification of Core and Fingerprint Proteins of Bacterial Genomes that Are Important for Plants.- Application of Integrated Computational Approaches in Prediction of Plant Virus Encoded miRNAs and Their Targeted Plant Genes.- Gene Co-Expression Network Analysis to Mine Genes Involved in Plant Secondary Metabolite Biosynthesis and Regulation.- Organic Supplementation of Vaccinium corymbosum Micropropagation Media.- Coffee Cell Suspensions as a Platform for Transient Gene Expression Analysis.- Agroinfiltration for Enhanced Transgene Expression in Coffee Leaves (Coffea arabica L.).- In Vitro Gamma Mutagenesis Techniques in Rice (Oryza sativa L. var. Lazarroz FL).- CRISPR/Cas9-Mediated Genome Editing in Indica Rice (Oryza sativa L. subsp. indica var. CR-5272).- Dual-Luciferase Reporter Assay for Pre-Screening CRISPR (d)Cas9-Mediated Epigenetic Editing on a Plant Promoter Using Human Cells.- In Silico Design of gRNA for CRISPR/Cas9-Mediated Gene Knock-Out.- CRISPR/Cas9 Vector Construction for Gene Knock-Out.- A Comprehensive Protocol for Assembly of Multiple gRNAs into a Direct Vector for Genome Editing in Tomato.- Genome Editing in Brassica juncea Using CRISPR/Cas9