Plant Kinases

Guide to the Book Plant Kinases.- The Age of Protein Kinases.- Expression and Purification of Active Protein Kinases from Wheat Germ Extracts.- Measurement of Plant Cyclin-Dependent Kinase Activity Using Immunoprecipitation-Coupled and Affinity Purification-Based Kinase Assays and the Baculovirus Expression System.- Mitogen-Activated Protein Kinase Activity and Reporter Gene Assays in Plants.- Use of Phospho-site Substitutions to Analyze the Biological Relevance of Phosphorylation Events in Regulatory Networks.- Bacterial Assay to Study Plant Sensor Histidine Kinases.- Substrate Analysis of Arabidopsis PP2C-type Protein Phosphatase.- Modulating and Monitoring MAPK Activity During Programmed Cell Death in Pollen.- Sensitizing Plant Protein Kinases to Specific Inhibition by ATP-Competitive Molecules.- Fluorescence Fluctuation Analysis of Receptor Kinase Dimerization.- Quantifying Degradation Rates of Trans-Membrane Receptor Kinases.- Fluorescence Correlation Spectroscopy and Fluorescence Recovery after Photobleaching to Study Receptor Kinase Mobility in planta.- Bimolecular-Fluorescence Complementation Assay to Monitor Kinase-Substrate Interactions in vivo.- Chemical Genetic Analysis of Protein Kinase Function in Plants.- Modified Metal Oxide Affinity Enrichment Combined with 2D-PAGE and Analysis of Phosphoproteomes.- Phosphoproteomics Using iTRAQ

From the reviews:"This book represents a compendium of practical protocols to experimentally address different aspects of plant kinase function and regulation. It is intended to provide a benchtop manual for newcomers to the field but also to present experimental alternatives for the more specialized and advanced user. ... this book compiles a wealth of information on how to study plant kinases and provides a useful set of instructions for a wide range of complementary techniques in this field." (Jörg Kudla, Journal of Plant Physiology, Vol. 169 (15), 2012)