Protein Chromatography

PrefaceDOWNSTREAM PROCESSING OF BIOTECHNOLOGY PRODUCTSIntroductionBioproducts and their ContaminantsBioprocessesRole of Chromatography in Downstream ProcessingReferencesINTRODUCTION TO PROTEIN CHROMATOGRAPHYIntroductionBasic Principles and DefinitionsModes of OperationPerformance FactorsSeparation Performance MetricsCHROMATOGRAPHY MEDIAIntroductionInteraction Types and ChemistryBuffers and Mobile PhasesPhysical Structure and PropertiesLABORATORY AND PROCESS COLUMNS AND EQUIPMENTIntroductionLaboratory-scale SystemsProcess Columns and EquipmentADSORPTION EQUILIBRIAIntroductionSingle Component SystemsMulti-component SystemsReferencesADSORPTION KINETICSIntroductionRate MechanismsBatch Adsorption KineticsDYNAMICS OF CHROMATOGRAPHY COLUMNSIntroductionConservation EquationsLocal Equilibrium DynamicsMulti-component SystemsDisplacement DevelopmentEFFECTS OF DISPERSION AND ADSORPTION KINETICS ON COLUMN PERFORMANCEIntroductionEmpirical Characterization of Column EfficiencyModeling and Prediction of Column EfficiencyGRADIENT ELUTION CHROMATOGRAPHYIntroductionGeneral Theory for Gradient Elution with Linear IsothermsLGE Relationships for Ion Exchange ChromatographyLGE Relationships for RPC and HICSeparations with pH GradientsModeling Gradient Elution with Non-linear IsothermsReferencesDESIGN OF CHROMATOGRAPHIC PROCESSESIntroductionChromatographic Process Steps and ConstraintsDesign for CaptureDesign for Chromatographic ResolutionSMB DesignReferencesINDEX

Giorgio Carta received his PhD in Chemical Engineering from the University of Delaware in 1984. Since then he has been a professor in the Department of Chemical Engineering at the University of Virginia, Charlottesville (USA), where his research focuses on transport phenomena and bioseparations. He regularly organizes professional courses on various aspects of bioseparations, including a course on protein chromatography development and scale-up together with Alois Jungbauer.Alois Jungbauer is the head of protein technology and downstream processing at the Department of Biotechnology of the University of Natural Resources and Applied Life Sciences in Vienna (Austria). For more than 20 years, Professor Jungbauer has worked in biochemical engineering, with a focus on biosparation, where he has published widely and holds 15 patents. For over 10 years, he has organized a biennial professional course in protein chromatography focused on mass transfer, dispersion, and scale-up.