Protein Chromatography

A Digest of Protein Purification.- Gel-Filtration Chromatography.- Immunoaffinity Chromatography.- Avoiding Proteolysis During Protein Chromatography.- Scale-Up of Protein Purification: Downstream Processing Issues.- Phage Display: A Powerful Technology for the Generation of High Specificity Affinity Reagents from Alternative Immune Sources.- Engineering Protein Stability.- Microfluidics in Protein Chromatography.- Tagging Recombinant Proteins to Enhance Solubility and Aid Purification.- Storage and Lyophilization of Pure Proteins.- Differential Precipitation and Solubilization of Proteins.- Ion-Exchange Chromatography: Basic Principles and Application to the Partial Purification of Soluble Mammalian Prolyl Oligopeptidase.- Protein Quantitation and Analysis of Purity.- Purification of Proteins Fused to Glutathione S-Tranferase.- Purification of Proteins Fused to Maltose-Binding Protein.- Purification of Proteins from Baculovirus-Infected Insect Cells.- Purification of Poly-Histidine-Tagged Proteins.- Immobilized Metal Affinity Chromatography/Reversed-Phase Enrichment of Phosphopeptides and Analysis by CID/ETD Tandem Mass Spectrometry.- Tag Removal by Site-Specific Cleavage of Recombinant Fusion Proteins.- Purification of Antibodies Using Affinity Chromatography.- Optimized Generation of High-Affinity, High-Specificity Single-Chain Fv Antibodies from Multi-Antigen Immunized Chickens.- Measuring Protein-Protein Interactions Using Biacore.- Ultra-Performance Liquid Chromatography-Mass Spectrometry of Proteins.- Hydrophobic Interaction Chromatography.- Fast Protein Liquid Chromatography.- Shotgun Proteomics: A Qualitative Approach Applying Isoelectric Focusing on Immobilized pH Gradient and LC-MS/MS.- Shotgun Proteomics: A Relative Quantitative Approach Using Off-Gel Electrophoresis and LC-MS/MS.- Clinical Proteomics: Liquid Chromatography-Mass Spectrometry (LC/MS) Purification Systems.- Strategies for the Purification of Membrane Proteins.- A Multi-Step