Proteomics for Biomarker Discovery

Affinity Depletion of Plasma and Serum for Mass Spectrometry-Based Proteome Analysis.- Tissue Sample Preparation for Biomarker Discovery.- Subcellular Fractionation for Identification of Biomarkers: Serial Detergent Extraction by Subcellular Accessibility and Solubility.- Analysis of Secreted Proteins.- Preparation of Human Cerebrospinal Fluid for Proteomics Biomarker Analysis.- Proteomic Analysis of Frozen Tissue Samples Using Laser Capture Microdissection.- Use of Formalin Fixed, Paraffin Embedded Tissue for Proteomic Biomarker Discovery.- Phosphopeptide Enrichment Using Offline Titanium Dioxide Columns for Phosphoproteomics.- iTRAQ-Labeling for Biomarker Discovery.- Analysis of Glycoproteins for Biomarker Discovery.- SILAC in Biomarker Discovery.- Trypsin-mediated 18O/16O Labeling for Biomarker Discovery.- Two-dimensional SDS-PAGE Fractionation of Biological Samples for Biomarker Discovery.- Informatics of Protein and Post-Translational Modification Detection via Shotgun Proteomics.- Quantitation of C-Met Tyrosine Phosphorylation Using MRM-MS.- Preparation of Human Serum for Prolactin Measurement by Multiple Reaction Monitoring Mass Spectrometry.- Label-Free Quantitative Shotgun Proteomics Using Normalized Spectral Abundance Factors.- Employment of Complementary Dissociation Techniques for Body Fluid Characterization and Biomarker Discovery.- DPhosphopeptide Microarrays for Comparative Proteomic Profiling of Cellular Lysates.- Tissue Preparation for MALDI MS Imaging of Protein and Peptides.- Plant Proteogenomics: From Protein Extraction to Improved Gene Predictions.- Label-free Differential Analysis of Murine Postsynaptic Densities.- Fractionation of Peptides by Strong Cation Exchange Liquid Chromatography.