Techniques in Confocal Microscopy

Böhnke
17. In Vivo Imaging of Mammalian Central Nervous System Neurons with the In Vivo Confocal Neuroimaging (ICON) Method
Sylvia Prilloff, Petra Henrich-Noack, Ralf Engelmann, Bernhard A. Sabel
18. Identification of Viral Infection by Confocal Microscopy
David N. Howell and Sara E. Miller
19. Membrane Trafficking
Sabine Kupzig, San San Lee, and George Banting

Part III. Green Fluorescent Protein
20. Monitoring of Protein Secretion with Green Fluorescent Protein
Christoph Kaether and Hans-Hermann Gerdes
21. Comparison of Enhanced Green Fluorescent Protein and Its Destabilized Form as Transcription Reporters
Xiaoning Zhao, Tommy Duong, Chiao-Chian Huang, Steven R. Kain, and Xianqiang Li
22. Measuring Protein Degradation with Green Fluorescent Protein
Stephen R. Cronin and Randolph Y. Hampton
23. Studying Nuclear Receptors with Green Fluorescent Protein Fusions
Gordon L. Hager
24. Signaling, Desensitization, and Trafficking of G Protein-Coupled Receptors Revealed by Green Fluorescent Protein Conjugates
Larry S. Barak, Jie Zhang, Stephen S. G. Ferguson, Stephane A. Laporte, and Marc G. Caron
25. Fluorescent Proteins in Single- and Multicolor Flow Cytometry
Lonnie Lybarger and Robert Chervenak
26. Jellyfish Green Fluorescent Protein: A Tool for Studying Ion Channels and Second-Messenger Signaling in Neurons
L. A. C. Blair, K. K.Bence, and J. Marshall
27. Expression of Green Fluorescent Protein and Inositol 1,4,5-Triphosphate Receptor in Xenopus laevis Oocytes
Atshushi Miyawaki, Julie M. Matheson, Lee G. Sayers, Akira Muto, Takayuki Michikawa, Teiichi Furuichi, and Katsuhiko Mikoshiba
28. Confocal Imaging of Ca2+, pH, Electrical Potential, and Membrane Permeability in Single Living Cells
John J. Lemasters, Donna R. Trollinger, Ting Qian, Wayne E. Cascio, and Hisayuki Ohata

Part IV. Laser Capture Microdissection
29. Laser Capture Microdissection and Its Applications in Genomics and Proteomics
James L. Wittliff
30. Going in Vivo with Laser Microdissection
Anette Mayer, Monika Stich, Dieter Brocksch, Karin Schütze, and Georgia Lahr
31. Flourescence in Situ Hybridization of LCM-Isolated Nuclei from Paraffin Sections
Douglas J. Demetrick, Sabita K. Murthy, and Lisa M. DiFrancesco

the National Board of Medical Examiners, including two years as chairman of the reproduction and endocrinology committee. The work of his laboratory has been recognized with a MERIT award from the NIH, the J.J. Abel Award of the American Society for Pharmacology and Experimental Therapeutics, the Weitzman, Oppenheimer and Ingbar Awards of the Endocrine Society, the National Science Medal of Mexico (the Miguel Aleman Prize) and the Stevenson Award of Canada. He is the recipient of the Oregon State Award for Discovery, the Media Award of the American College of Neuropsychopharmacology and was named a distinguished Alumnus of Baylor College of Medicine in 2012. Conn is a previous member of Council for the American Society for Cell Biology and the Endocrine Society and is a prior President of the Endocrine Society, during which time he founded the Hormone Foundation and worked with political leadership to heighten the public's awareness of diabetes. Conn's students and fellows have gone on to become leaders in industry and academia. He is an elected member of the Mexican Institute of Medicine and a fellow of the American Association for the Advancement of Science. He is the co-author of The Animal Research War (2008) and many articles for the public and academic community on the value of animal research and the dangers posed by animal extremism. His op/eds have appeared in The Washington Post, The LA Times, The Wall Street Journal, the Des Moines Register, and elsewhere. Conn consults with organizations that are influenced by animal extremism and with universities and companies facing challenges from these groups.