RNA-Protein Interaction Protocols

1. Isolation of a Sequence-Specific RNA Binding Protein, Polypyrimidine Tract Binding Protein using RNA Affinity Chromatography Shalini Sharma 2 An Affinity Oligonucleotide Displacement Strategy to Purify Ribonucleoprotein Complexes Applied to Human Telomerase Isabel Kurth, Gaël Cristofari, and Joachim Lingner 3 RNA Affinity Tags for the Rapid Purification and Investigation of RNAs and RNA-Protein Complexes Scott C. Walker, Felicia H. Scott, Chatchawan Srisawat, and David R. Engelke 4 Assembly and Glycerol Gradient Isolation of Yeast Spliceosomes Containing Transcribed or Synthetic U6 SnRNA Ken Dery, Shyue-Lee Yean, and Ren-Jang Lin 5 Purification of Ribonucleoproteins Using Peptide-Elutable Antibodies and Other Affinity Techniques Scott Stevens 6 CLIP: Cross-Linking and ImmunoPrecipitation of In Vivo RNA Targets of RNA Binding Proteins Kirk B Jensen and Robert B. Darnell 7 Quantitative Analysis of Protein-RNA Interactions by Gel Mobility Shift Sean P. Ryder, Michael I. Recht, and James R. Williamson 8 Monitoring Assembly of Ribonucleoprotein Complexes by Isothermal Titration Calorimetry Michael I. Recht, Sean P. Ryder, and James R. Williamson 9 Characterization of RNA-Protein Interactions by Phosphorothioate Footprinting and Its Applications to the Ribosome A. Ozlem Tastan Bishop, Ulrich Stelzl, Markus Pech, and Knud H. Nierhaus 10 In vivo Analysis of Ribonucleoprotein Complexes Using Nucleotide Analog Interference Mapping Lara Weinstein Szewczak 11 T7 RNA Polymerase Mediated Incorporation of 8-N3AMP into RNA for Studying Protein-RNA Interactions Rajesh Gaur 12 A Simple Crosslinking Method, CLAMP, to Map the Sites of RNA Contacting Domains within A Protein Hiren Banerjee and Ravinder Singh 13 Proteins Specifically Modified with a Chemical Nuclease as Probes ofRNA-Protein Interaction Oliver A. Ken and Andrew M. MacMillan 14 RNA-Protein Crosslink Mapping using TEV Protease Ian A. Turner, Chris M. Norman, Mark J. Churcher, and Andrew J. Newman 15 Structural Analysis

From the reviews of the second edition:"Detailed protocols for analysis of specific examples of RNA protein interactions are described. ... Anyone studying RNA protein interactions should seriously consider reading this book, not only as a technical reference, but also as a source of ideas. ... It's a book for the lab bench and the PIs office. Highly recommended." (Laurence Tiley, Microbiology Today, February, 2009)"The second edition of RNA-protein interaction protocols is part of the Methods in Molecular Biology series and includes detailed methods covering a wide range of approaches to characterise RNA-protein interactions. ... This book ... is well written, well indexed and well referenced. Each protocol is described in enough detail to be followed by an experienced user. For an RNA-based laboratory this book provides an excellent description of the latest techniques with particular attention given to manipulation of RNPs from in vivo sources." (Matthew Bentham, British Toxicology Society Newsletter, June-August, 2009)