TGF-beta Signaling

Regulation of TGF-b Receptors.- Determining TGF-beta Receptor Levels in the Cell Membrane.- Post-translational Modifications of TGF-b Receptors.- Production, Isolation and Structural Analysis of Ligands and Receptors of the TGF-b Superfamily.- Phosphorylation of Smads by Intracellular Kinases.- Analysis of Smad Phosphatase Activity in vitro.- Three-dimensional Mammary Epithelial Cell Morphogenesis Model for Analysis of TGF-b Signaling.- TGF-beta Signaling in Stem Cell Regulation.- Analysis of Epithelial-Mesenchymal Transition Induced by Transforming Growth Factor beta.- In vitro Th differentiation protocol.- Interrogating TGF-b Function and Regulation in Endothelial Cells.- Isolation and Manipulation of Adipogenic Cells to Assess TGF-b Superfamily Functions.- Imaging TGFb Signaling in Mouse Models of Cancer Metastasis.- Generation and Characterization of Smad7 Conditional Knockout Mice.- Monitoring Smad Activity in vivo Using the Xenopus Model System.- Animal Cap Assay for TGF-b Signaling.- Detection of Smad Signaling in Zebrafish Embryos.- Role of TGF-beta signaling in Coupling Bone Remodeling.- Studying the Functions of TGF-beta Signaling in the Ovary.- Quantitative Real-Time PCR Analysis of MicroRNAs and Their Precursors Regulated byTGF-b Signaling.- TGF-beta Regulated microRNAs and Their Function in Cancer Biology.- Epigenomic Regulation Of Smad1 Signaling During Cellular Senescence Induced by Ras Activation.- The Role of Ubiquitination to Determine Non-Smad Signaling Responses.- Genome-wide RNAi Screening to Dissect the TGF-b Signal Transduction Pathway.- Measuring TGF-beta Ligand Dynamics in Culture Medium.