Viral Metagenomics

Host-Associated Bacteriophage Isolation and Preparation for Viral Metagenomics.- SmallRNAs Isolation from Tissues of Grapevine and Woody Plants.- Double Stranded RNA-Enriched Preparations to Identify Viroids by Next Generation Sequencing.- Viral Double-Stranded RNAs (dsRNAs) from Plants: Alternative Nucleic Acid Substrates for High-Throughput Sequencing.- Work-Up of Human Blood Samples for Deep Sequencing of HIV-1 Genomes.- Monolith Chromatography as Sample Preparation Step in Virome Studies of Water Samples.- Viral Metagenomics Approaches for High-Resolution Screening of Multiplexed Arthropod and Plant Viral Communities.- Different Approaches to Discover Mycovirus Associated to Marine Organisms.- Use of siRNAs for Diagnosis of Viruses Associated to Woody Plants in Nurseries and Stock Collections.- The Use of High Throughput Sequencing for the Study and Diagnosis of Plant Viruses and Viroids in Pollen.- High-Resolution Screening ofViral Communities and Identification of New Pathogens in Fish Using Next Generation Sequencing.- Metagenomic Analyses of the Viruses Detected in Mycorrhizal Fungi and their Host Orchid.- DNA Multiple Sequence Alignment Guided by Protein Domains: The MSA-PAD 2.0 Method.- From Whole Genome Shotgun Sequencing to Viral Community Profiling: The ViromeScan Tool.- Shannon Entropy to Evaluate Substitution Rate Variation among Viral Nucleotide Positions in Datasets of Viral siRNAs.- Insect Virus Discovery by Metagenomic and Cell Culture-Based Approaches.