Wound Healing
Methods and Protocols
(Sprache: Englisch)
During the past decade, significant progress in molecular and cellular te- niques has greatly advanced our understanding of the wound healing p- cess. Many of these new techniques have been utilized in the context of more classic models of wound healing....
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Klappentext zu „Wound Healing “
During the past decade, significant progress in molecular and cellular te- niques has greatly advanced our understanding of the wound healing p- cess. Many of these new techniques have been utilized in the context of more classic models of wound healing. The combination of new and classic approaches has allowed scientists to make exciting discoveries in the field of tissue repair, resulting in an explosion of information about the healing p- cess. Importantly, these new findings have great relevance beyond wound healing itself. The injury repair process cuts across many disciplines, exte- ing to such broad fields as cancer, inflammation, and atherosclerosis. The relevance of the field to these many disciplines has generated great interest in models and methods for the study of wound healing. The goal of Wound Healing: Methods and Protocols is to provide scientists from many dis- plines with a compendium of classic and contemporary protocols from r- ognized experts in the field of wound healing. We hope this volume will be useful not only to those working within the field itself, but also to scientists from other disciplines who wish to adapt wound healing models to their own experimental needs. The process of wound healing encompasses many different biologic p- cesses, including epithelial growth and differentiation, fibrous tissue prod- tion and function, angiogenesis, and inflammation.
Inhaltsverzeichnis zu „Wound Healing “
Experimental Models of Wound Healing.- Excisional Wound Healing.- Methods in Reepithelialization.- Incisional Wound Healing.- Animal Models of Ischemic Wound Healing.- Corneal Injury.- Subcutaneous Sponge Models.- A Mouse Model of Burn Wounding and Sepsis.- A Porcine Burn Model.- Wound Healing in Airways In Vivo.- Murine Models of Intestinal Anastomoses.- Murine Model of Peritoneal Adhesion Formation.- Methods for Investigating Fetal Tissue Repair.- Growth of Human Blood Vessels in Severe Combined Immunodeficient Mice.- Tissue Repair in Models of Diabetes Mellitus.- Wound Healing Studies in Transgenic and Knockout Mice.- Wound Repair in Aging.- Specimen Collection and Analysis.- Suction Blister Model of Wound Healing.- Implantable Wound Healing Models and the Determination of Subcutaneous Collagen Deposition in Expanded Polytetrafluoroethylene Implants.- The Fibroblast-Populated Collagen Lattice.- A Quantifiable In Vitro Model to Assess Effects of PAI-1 Gene Targeting on Epithelial Cell Motility.- Human Skin Organ Culture.- In Vitro Matrigel Angiogenesis Model.- Analysis and Manipulation of Wound Healing.- Quantification of Wound Angiogenesis.- In Vivo Matrigel Migration and Angiogenesis Assays.- Endothelial Cell Migration Assay.- Analysis of Collagen Synthesis.- Method for Detection and Quantitation of Leukocytes During Wound Healing.- Detection of Reactive Oxygen Intermediate Production by Macrophages.- Measurement of Chemokines at the Protein Level in Tissue.- Methods of Measuring Oxygen in Wounds.- Isolation, Culture, and Characterization of Human Intestinal Smooth Muscle Cells.- Use of High-Throughput Microarray Membranes for cDNA Analysis of Cutaneous Wound Repair.- Particle-Mediated Gene Therapy of Wounds.
Bibliographische Angaben
- 2010, Softcover reprint of hardcover 1st ed. 2003, XVI, 468 Seiten, Maße: 15,3 x 23 cm, Kartoniert (TB), Englisch
- Herausgegeben: Luisa A. DiPietro, Aime L. Burns
- Verlag: Springer, Berlin
- ISBN-10: 161737296X
- ISBN-13: 9781617372964
Sprache:
Englisch
Pressezitat
From the reviews: "Clinical and basic science researchers with an interest in wound healing of various kinds are an appropriate audience for this work. Editors and authors represent some of the leading wound healing laboratories in the United States with a smaller number of presentations from overseas. . .Chapters are written in a straightforward manner. . .This book is a collection of techniques and insights which belongs on the shelf of laboratories or research organizations having an interest in wounds." -Doody's Health Sciences Book Review Journal
"This informative volume provides a highly practical collection of widely used model systems and methods for studying the injury repair process and is therefore highly recommended as a valuable reference source for all individuals with interests in wound healing." - Carbohydrate Polymers
"The editors have done an impressive job of editing the book into both a practical and readable style. It is highly recommended for any group that wishes to participate in this exciting and expanding field of research." -British Journal of Surgery
"This book ... provides a range of classical and more recent tools in wound research. ... an impressive collection of both animal and in vitro-based techniques useful for experimental studies of tissue damage and repair. ... Each chapter has a concluding notes section offering valuable suggestions and warnings based on practical experience. The book is highly recommended for all basic and clinical researchers involved in tissue repair biology and particularly those with an interest in experimental wound modelling and analysis." (Ola Rollman, Acta Dermato-Venereologica, Vol. 85, 2005)
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