Assay Development (PDF)
Fundamentals and Practices
(Sprache: Englisch)
Essential principles and practice of assay development
The first comprehensive, integrated treatment of the subject,
Assay Development: Fundamentals and Practices covers the essentials
and techniques involved in carrying out an assay project in...
The first comprehensive, integrated treatment of the subject,
Assay Development: Fundamentals and Practices covers the essentials
and techniques involved in carrying out an assay project in...
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Essential principles and practice of assay development
The first comprehensive, integrated treatment of the subject,
Assay Development: Fundamentals and Practices covers the essentials
and techniques involved in carrying out an assay project in either
a biotechnology/drug discovery setting or a platform setting.
Rather than attempting comprehensive coverage of all assay
development technologies, the book introduces the most widely used
assay development technologies and illustrates the art of assay
development through a few commonly encountered biological targets
in assay development (e.g., proteases, kinases, ion channels, and G
protein-coupled receptors). Just enough biological background for
these biological targets is provided so that the reader can follow
the logics of assay development. Chapters discuss:
* The basics of assay development, including foundational concepts
and applications
* Commonly used instrumental methods for both biochemical assays
and cell-based assays
* Assay strategies for protein binding and enzymatic activity
* Cell-based assays
* High-throughput screening
An in-depth study of the now popular Caliper's off-chip kinase
assay provides an instructive, real-world example of the assay
development process.
The first comprehensive, integrated treatment of the subject,
Assay Development: Fundamentals and Practices covers the essentials
and techniques involved in carrying out an assay project in either
a biotechnology/drug discovery setting or a platform setting.
Rather than attempting comprehensive coverage of all assay
development technologies, the book introduces the most widely used
assay development technologies and illustrates the art of assay
development through a few commonly encountered biological targets
in assay development (e.g., proteases, kinases, ion channels, and G
protein-coupled receptors). Just enough biological background for
these biological targets is provided so that the reader can follow
the logics of assay development. Chapters discuss:
* The basics of assay development, including foundational concepts
and applications
* Commonly used instrumental methods for both biochemical assays
and cell-based assays
* Assay strategies for protein binding and enzymatic activity
* Cell-based assays
* High-throughput screening
An in-depth study of the now popular Caliper's off-chip kinase
assay provides an instructive, real-world example of the assay
development process.
Autoren-Porträt von Ge Wu
Ge Wu, PhD, is Director of Assay Development at IrisInternational. He obtained his doctorate in biochemistry from UCLA,
performed postdoctoral work at Harvard Medical School, and was a
member of the faculty at Cornell University Medical College. Prior
to joining Iris International, he worked at Merck & Co.,
Caliper Technologies, Amphora Discovery, and FivePrime
Therapeutics. He is the founding Director of Assay Development and
Screening at FivePrime Therapeutics. Dr. Wu is a leading figure in
assay development and screening in biopharmaceutical discovery
using primary cell assay and the author of an acclaimed review in
this subject.
Bibliographische Angaben
- Autor: Ge Wu
- 2010, 1. Auflage, 448 Seiten, Englisch
- Verlag: John Wiley & Sons
- ISBN-10: 0470583118
- ISBN-13: 9780470583111
- Erscheinungsdatum: 20.07.2010
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- Größe: 8.36 MB
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Sprache:
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