Genetic knockout of Cathepsin D using zinc-finger nucleases delivered by AAV vectors (PDF)
(Sprache: Englisch)
Project Report from the year 2013 in the subject Biology - Neurobiology, grade: 1.0, University of Göttingen, language: English, abstract: Genetic engineering is known as a powerful technique for basic research and clinical applications. Recent progress in...
sofort als Download lieferbar
eBook (pdf)
13.99 €
- Lastschrift, Kreditkarte, Paypal, Rechnung
- Kostenloser tolino webreader
Produktdetails
Produktinformationen zu „Genetic knockout of Cathepsin D using zinc-finger nucleases delivered by AAV vectors (PDF)“
Project Report from the year 2013 in the subject Biology - Neurobiology, grade: 1.0, University of Göttingen, language: English, abstract: Genetic engineering is known as a powerful technique for basic research and clinical applications. Recent progress in development of zinc-finger nucleases (ZFNs), which combine the DNA cleavage ability of Fok1 restriction enzyme with highly specific recognition properties of zinc-finger motifs, allows to improve efficiency and to broaden the field of use of genome editing. Here, we demonstrate our initial results in generating novel tools for Cathepsin D gene knockout in neurons based on ZFNs technology and mediated by adeno-associated virus (AAV) vectors. Pairs of AAV-ZFNs were produced and demonstrated the robust expression of nucleases in neuronal cell culture. Observed toxicity most likely was associated with heterodimerization but not homodimerization of ZFNs; cytotoxicity was greatly reduced when ZFN were provided at lower concentrations. Future studies evaluating efficiency of Ctsd knockout, off-target effects on molecular level and long-term outcomes in vivo can be performed.
Bibliographische Angaben
- Autor: Maryna Psol
- 2013, 1. Auflage, 19 Seiten, Englisch
- Verlag: GRIN Verlag
- ISBN-10: 3656524688
- ISBN-13: 9783656524687
- Erscheinungsdatum: 25.10.2013
Abhängig von Bildschirmgröße und eingestellter Schriftgröße kann die Seitenzahl auf Ihrem Lesegerät variieren.
eBook Informationen
- Dateiformat: PDF
- Größe: 2.26 MB
- Ohne Kopierschutz
- Vorlesefunktion
Sprache:
Englisch
Kommentar zu "Genetic knockout of Cathepsin D using zinc-finger nucleases delivered by AAV vectors"
0 Gebrauchte Artikel zu „Genetic knockout of Cathepsin D using zinc-finger nucleases delivered by AAV vectors“
| Zustand | Preis | Porto | Zahlung | Verkäufer | Rating |
|---|
Schreiben Sie einen Kommentar zu "Genetic knockout of Cathepsin D using zinc-finger nucleases delivered by AAV vectors".
Kommentar verfassen