High Content Screening (PDF)

The authoritative reference on HCS in biological and pharmaceutical research

High Content Screening (HCS) has been a leading methodology in toxicology studies for years. Recent advances have broadened the range of applications to encompass new areas. High Content Screening: Science, Techniques, and Applications provides comprehensive coverage of HCS in four sections:

* The basics of HCS, from the definition to detailed discussions of component technologies

* Examples of HCS used in biological applications and early drug discovery, with an emphasis on applications in oncology and neuroscience

* The use of HCS across the drug development pipeline

* Data management, data analysis, and systems biology, with guidelines for using the large datasets generated by HCS in systems-level studies

With chapters contributed by leading authorities from academia and industry, this guide covers:

* A wide range of topics, including assay development, cell culture, image processing, robotics, database architecture and management, model systems for analysis, and more

* Focused discussions on imaging in 3D, imaging of tissues for pharmacodynamic studies, and screening of both small molecule and RNAi libraries by HCS

* The roles of bench researchers and IT personnel in implementing and maintaining HCS platforms

* The challenges and advantages of using HCS today, and a look at future directions

With eighty-seven detailed figures readers can refer to in full color on the accompanying CD-ROM, this is the premier, hands-on reference on HCS for researchers in academia, biotechnology, and pharmaceutical companies. It's also an excellent resource for lab managers and graduate students in biochemistry, cell biology, toxicology, and related fields.