Proteomics Today / Wiley-Interscience Series on Mass Spectrometry (PDF)

PREFACE TO PART I. ACKNOWLEDGMENT. I: INTRODUCTION TO PART I. 1. INSTRUMENTATION AND DEVELOPMENTS. 1.1 Introduction. 1.2 Ionization Techniques for Macromolecules. 1.3 Examples on Analytical Solutions Based on FAB-MS. 1.4 Electrospray Ionization. 1.5 Matrix-Assisted Laser Desorption Ionization. 1.6 Ion Detection. 1.7 Types of Analyzers. 1.8 Hybrid Analyzers. 1.9 Tandem Mass Spectrometry. 1.10 Current MS Instrumentation in Proteome Analyses. 1.11 Current MS-Based Proteomics. 1.12 Recent Achievements and Future Challenges. 1.13 Concluding Remarks. References. 2. PROTEOMICS IN CANCER RESEARCH. 2.1 Introduction. 2.2 Pancreatic Ductal Adenocarcinoma. 2.3 Proteomic Analysis of Human Breast Carcinoma. 2.4 Proteomic Profiling of Chemoresistant Cancer Cells. 2.5 Signal Pathway Profiling of Prostate Cancer. 2.6 Emerging Role of Functional and Activity-Based Proteomics in Disease Understanding. 2.7 Activity-Based Protein Profiling. 2.8 Probing Protein Functions Using Chromophore-Assisted Laser Inactivation. 2.9 Role of Protein-Tyrosine Kinases. 2.10 Concluding Remarks and Future Prospects. References. 3. CURRENT STRATEGIES FOR PROTEIN QUANTIFICATION. 3.1 Introduction. 3.2 Global Internal Standard Technology. 3.3 Differential In-Gel Electrophoresis. 3.4 Quantification of Modified Proteins. 3.5 Comments and Considerations. 3.6 Other Approaches. 3.7 Emerging Role of Microfluidic Devices. 3.8 Concluding Remarks. References. II: PROTEOMICS TODAY: SEPARATION SCIENCE AT WORK. 4. CONVENTIONAL ISOELECTRIC FOCUSING IN GEL MATRICES AND CAPILLARIES AND IMMOBILIZED pH GRADIENTS. 4.1 Introduction. 4.2 Conventional Isoelectric Focusing in Amphoteric Buffers. 4.3 Immobilized pH Gradients. 4.4 Capillary Isoelectric Focusing. 4.5 Separation of Peptides and Proteins by CZE in Isoelectric Buffers. 4.6 Conclusions. References. 5. SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL ELECTROPHORESIS. 5.1 Introduction. 5.2 SDS-Protein Complexes: a Refinement of the Model. 5.3 Theoretical Background of Mr

MAHMOUD HAMDAN, PhD, is the head of Mass Spectrometry &
Separation Technologies, GlaxoSmithKline, Verona, Italy.

PIER GIORGIO RIGHETTI, PhD, is Professor of Biochemistry and
director of the Laboratory of Proteome Science at the University of
Verona, Italy.

Dieses eBook können Sie uneingeschränkt auf allen Geräten der tolino Familie lesen. Zum Lesen auf sonstigen eReadern und am PC benötigen Sie eine Adobe ID.