Software-Assisted Method Development in High Performance Liquid Chromatography (ePub)

This handbook gives a general overview of the possibilities in recent developments in chromatographic retention modeling. As a result of the latest developments in modeling software, several new features are now accessible, opening a new level in HPLC method development.

Many of these current possibilities in software assisted liquid chromatographic method modeling for analytical purposes are presented. Several modes of chromatography, including Reversed-Phase Liquid Chromatography (RPLC), Ion Exchange Chromatography (IEX), Hydrophobic Interaction Chromatography (HIC), and Hydrophilic Interaction Liquid Chromatography (HILIC) are explained in detail. For all these chromatographic modes, the most important variables for tuning retention and selectivity are exposed.

Beside the industrial and practical benefits of retention modeling, the possibilities in teaching and education are also illustrated. Finally, numerous representative industrial examples are shown, to highlight the benefits, time and cost savings offered by state-of-the-art software assisted HPLC method development.

• Preface
• About the Authors
• List of Abbreviations and Symbols
• Introduction: The First Steps of Method Development in Liquid Chromatography (Imre Molnár and Szabolcs Fekete)
• HPLC Method Development by QbD Compatible Resolution Modeling (DryLab4) (Szabolcs Fekete, Imre Molnár, Hans-Jürgen Rieger and Róbert Kormány)
• ChromSword^®: Software for Method Development in Liquid Chromatography (Sergey V Galushko, Irina Shishkina, Evalds Urtans and Oksana Rotkaja)
• Intelligent Systems to Predict Retention from Molecular Properties for Reversed-Phase HPLC Separations (György Morovján)
• Statistical Methods in Quality by Design Approach to Liquid Chromatography Methods Development (Hermane T Avohou, Cédric Hubert, Benjamin Debrus, Pierre Lebrun, Serge Rudaz, Bruno Boulanger and Philippe Hubert)
• Optimization of Peak Capacity (Krisztián Horváth)
• 'HPLC Teaching Simulator': A Simple Excel Tool for Teaching Liquid Chromatography (Davy Guillarme and Jean-Luc Veuthey)
• Examples on Small Molecule Pharmaceuticals (From the Beginning to the Validation) (Róbert Kormány and Norbert Rácz)
• Computer-Assisted Method Development in Characterization of Therapeutic Proteins by Reversed-Phase Chromatography (Szabolcs Fekete)
• Computer-Assisted Method Development in Characterization of Therapeutic Proteins by Ion-Exchange Chromatography (Szabolcs Fekete)
• Computer-Assisted Method Development in Characterization of Therapeutic Proteins by Hydrophobic Interaction Chromatography (Balazs Bobaly and Szabolcs Fekete)
• Computer-Assisted Method Development in Characterization of Therapeutic Proteins by Hydrophilic Interaction Liquid Chromatography (Szabolcs Fekete and Balazs Bobaly)
• Index

• All these chapters are written by recognized experts in their fields and therefore, this book is recommended for every chromatographers who want to save time, costs and efforts in method development and want to understand the retention behavior of their compounds