Stem Cell Bioprocessing (ePub)
For Cellular Therapy, Diagnostics and Drug Development
(Sprache: Englisch)
Stem cell bioprocessing describes the main large-scale bioprocessing strategies for both stem cell culture and purification, envisaging the application of these cells for regenerative medicine and drug screening. Bioreactor configurations are described,...
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Stem cell bioprocessing describes the main large-scale bioprocessing strategies for both stem cell culture and purification, envisaging the application of these cells for regenerative medicine and drug screening. Bioreactor configurations are described, including their applications for stem cell expansion, and stem cell separation techniques such as isolation and purification are discussed. Basic definitions are provided concerning the different types of stem cells, from adult stem cells to the more recent induced pluripotent stem cells. The main characteristics of these different stem cell types are described, alongside the molecular mechanisms underlying their self-renewal and differentiation. The book also focuses on methodologies currently used for in vitro stem cell culture under static conditions, including the challenge of xeno-free culture conditions, as well as culture parameters that influence stem cell culture. Approaches for both stem cell culture and separation in micro-scale conditions are presented, including the use of cellular microarrays for high-throughput screening of the effect of both soluble and extracellular matrix molecules. A further section is dedicated to application of stem cells for regenerative medicine.
- Maintains a unique focus on both the basic stem cell biology concepts, and their translation to large-scale bioprocessing approaches
- Envisages the use of stem cells in regenerative medicine and drug screening applications
- Discusses the application of microscale techniques as a tool to perform basic stem cell biology studies
Autoren-Porträt von Tiago G. Fernandes, M. Margardia Diogo, Joaquim M. S. Cabral
Dr Tiago G. Fernandes is currently a Post-doctoral research fellow at the Institute for Biotechnology and Bioengineering, Lisbon, Portugal. His research interests are focused on the development of artificial cellular niches for studying the mechanisms that affect human stem cell pluripotency, and the cellular and molecular events that regulate stem cell functions. During his doctoral studies he also worked at the Rensselaer Polytechnic Institute, NY, USA, in the development of microscale platforms for high-throughput studies of stem cell fate.
Bibliographische Angaben
- Autoren: Tiago G. Fernandes , M. Margardia Diogo , Joaquim M. S. Cabral
- 2013, 236 Seiten, Englisch
- Verlag: Elsevier Science & Techn.
- ISBN-10: 1908818301
- ISBN-13: 9781908818300
- Erscheinungsdatum: 15.11.2013
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eBook Informationen
- Dateiformat: ePub
- Größe: 2.50 MB
- Mit Kopierschutz
- Vorlesefunktion
Sprache:
Englisch
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Pressezitat
"This book offers an interesting review of the current research and advances in the field of stem cell biology. It focuses on unique and complex requirements and challenges of stem cell culture and seems to target audience of stem cell researchers, but the well-written introduction to basic stem cell biology makes it accessible to a wider audience."--Doody.com, January 3, 2014
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