A Practical Guide to Protein and Peptide Purification for Microsequencing
Why a Second Edition?
The Second Edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather than the more specific question asked in the first edition, "How can I obtain the...
The Second Edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather than the more specific question asked in the first edition, "How can I obtain the...
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Why a Second Edition?The Second Edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather than the more specific question asked in the first edition, "How can I obtain the N-terminal sequence?" Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry.
Bibliographische Angaben
- Herausgegeben:Matsudaira, Paul T.
- Verlag: Academic Press
- EAN: 9780124802827
Inhaltsverzeichnis zu „A Practical Guide to Protein and Peptide Purification for Microsequencing “
Strategies for Obtaining Partial Amino Acid Sequence Data from Small Quantities (5nmol) of Pure of Partially Purified Protein. Enzymatic Digestion of Proteins and HPLC Peptide IsolationPurification of Proteins and Peptides By SDS-PAGE
Internal Amino Acid Sequence Analysis of Proteins After in Situ Protease Digestion on Nitrocellulose
Mass Spectrometric Strategies for the Structural Characterization of Proteins
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