Carotenoids: Carotenoid and Apocarotenoid Biosynthesis, Metabolic Engineering and Synthetic Biology

1. Metabolomics-based analysis of carotenoids and related metabolites in various species via quantitative trait loci and genome wide association mapping approaches
2. Enzymatic isomerization of ?-carotene mediated by the heme-containing isomerase Z-ISO
3. Using bacteria for functional analysis of genes encoding carotenoid biosynthetic enzymes
4. Rice Callus as a High Throughput Platform for Synthetic Biology and Metabolic Engineering of Carotenoids
5. Transient expression in Nicotiana benthamiana: A simple platform to investigate genes encoding carotenoid biosynthesis enzymes from diverse algal lineages
6. Protein-protein interaction techniques to investigate post-translational regulation of carotenogenesis
7. The isolation of sub-chromoplast structures from tomato and capsicum fruit
8. Carrot protoplasts as a suitable method for protein subcellular localization
9. High throughput production and characterization of carotenoid enzymes for structural and functional studies
10. Production and structural characterization of the cytochrome P450 enzymes in carotene ring hydroxylation
11. Characterizing CYTOCHROME P450 Enzymes Involved in Plant Apocarotenoid Metabolism by Using Engineered Yeast System
12. Preparation of carotenoid cleavage dioxygenases for crystallography
13. Heme-dependent enzymes involved in carotenoids biosynthesis in plants
14. Tracking sub-plastidic localization of carotenoid metabolic enzymes with proteomics
15. Golden Rice and lessons learned for inspiring future metabolic engineering strategies and synthetic biology solutions
16. Genetic transformation of Cassava to improve carotenoids: a protocol revisited
17. Elevating fruit carotenoid content in apple (Malus x domestica Borkh)
18. Engineering microbial synthesis of carotenoids
19. The breeder's tool-box for enhancing the content of esterified carotenoids in wheat: from extraction and profiling of carotenoids to marker-assisted selection of candidate genes
20. Drawing on enzyme