Cilia: Model Organisms and Intraflagellar Transport

Stephen M. King is Professor of Molecular Biology and Biophysics at the University of Connecticut School of Medicine and is also director of the electron microscopy facility. He has studied the structure, function and regulation of dyneins for over 30 years using a broad array of methodologies including classical/molecular genetics, protein biochemistry, NMR structural biology and molecular modeling, combined with cell biological approaches, imaging and physiological measurements.

1. Manipulating Ciliary Protein-Encoding Genes in Tetrahymena thermophilaDrashti Dave, Dorota Wloga, and Jacek Gaertig2. Approaches for Functional Analysis of Flagellar Proteins in African TrypanosomesMichael Oberholzer, Miguel A. Lopez, Katherine S. Ralston, and Kent L. Hill3. Tools for Analyzing Intraflagellar Transport in TrypanosomesDaria Julkowska and Philippe Bastin4. Schmidtea mediterranea: A Model System for Analysis of Motile CiliaPanteleimon Rompolas, Ramila S. Patel-King, and Stephen M. King5. Targeted Gene Silencing by RNA Interference in ChlamydomonasEun-Jeong Kim and Heriberto Cerutti6. Analysis of Cargo Transport by IFT and GFP Imaging of IFT in ChlamydomonasDennis Diener7. Genetic and Phenotypic Analysis of Flagellar Assembly Mutantsin Chlamydomonas reinhardtiiCarlo Iomini, Jacob E. Till, and Susan K. Dutcher8. Recording and Analyzing IFT in Chlamydomonas FlagellaWilliam Dentler, Kristyn VanderWaal, and Mary E Porter9. Total Internal Reflection Fluorescence (TIRF) Microscopyof Chlamydomonas FlagellaBenjamin D. Engel, Karl-Ferdinand Lechtreck, Tsuyoshi Sakai, Mitsuo Ikebe, George B. Witman, andWallace F. Marshall10. Purification of IFT Particle Proteins and Preparation of RecombinantProteins for Structural and Functional AnalysisRobert H. Behal, Ewelina Betleja, and Douglas G. Cole11. Studying Cilia in ZebrafishIain Drummond12. Analysis of IFT Kinesins in Developing Zebrafish Cone PhotoreceptorSensory CiliaChristine Insinna, Katherine Luby-Phelps, Brian A. Link, and Joseph C. Besharse13. Analysis of Intraflagellar Transport in C. elegans Sensory CiliaLimin Hao, Seyda Acar, James Evans, Guangshuo Ou, and Jonathan M. Scholey14. Functional Genomics of IntraflagellarTransport-Associated Proteins in C. elegansPeter N. Inglis, Oliver E. Blacque, and Michel R. Leroux15. Generating Conditional Mutants to Analyze Ciliary Functions:The Use of Cre-Lox Technology to Disrupt Cilia in Specific OrgansAmber K. O'Connor, Robert A. Kesterson, and Bradley K. Yoder16.