Tumor Immunology and Immunotherapy - Cellular Methods Part A

1. Cytokine profiling of tumor-infiltrating T lymphocytes by flow cytometry Jeremy B. Foote, Sujith Sarvesh and Leisha A. Emens 2. High-throughput identification of human antigen-specific CD8+ and CD4+ T cells using soluble pMHC multimers Morgane Magnin, Philippe Guillaume, George Coukos, Alexandre Harari and Julien Schmidt 3. In vitro assays for effector T cell functions and activity of immunomodulatory antibodies Roberta Zappasodi, Sadna Budhu, Mohsen Abu-Akeel and Taha Merghoub 4. A cytofluorimetric assay to evaluate T cell polyfunctionality Belinda Palermo, Mariangela Panetta, Giulia Campo and Paola Nisticò 5. Assessment of memory formation by metabolically engineered antigen-specific CD8 T cells Mathias Wenes, Pedro Romero and Lianjun Zhang 6. Functional characterization of tumor antigen-specific T-cells isolated from the tumor microenvironment of sleeping beauty induced murine glioma models Mahmoud S. Alghamri, Felipe J. Núñez, Neha Kamran, Stephen Carney, David Altshuler, Pedro R. Lowenstein and Maria G. Castro 7. Methods to edit T cells for cancer immunotherapy Francesca Lucibello, Silvia Menegatti and Laurie Menger 8. Generating stem-like memory T cells with antioxidants for adoptive cell transfer immunotherapy of cancer Karolina Pilipow, Eloise Scamardella and Enrico Lugli 9. Reverse immunology: From peptide sequence to tumor-killing human T-cell clones Christophe Vanhaver, Monica Gordon-Alonso, Alexandre Bayard, Maria Teresa Catanese, Didier Colau, Pierre van der Bruggen and Annika M. Bruger 10. Generation of TCR-engineered reference cell samples to control T-cell assay performance Nicole Bidmon, Cécile Gouttefangeas and Sjoerd H. van der Burg 11. In vitro expansion of V?9Vd2 T cells for immunotherapy Christian Peters, Léonce Kouakanou, Hans-Heinrich Oberg, Daniela Wesch and Dieter Kabelitz 12. CFSE dilution to study human T and NK cell proliferation in vitro Iñigo Terrén, Ane Orrantia, Joana Vitallé, Olatz Zenarruzabeitia and Francisco Borrego 13.