2D PAGE: Sample Preparation and Fractionation

Sample Preparation and Fractionation: Volume 1 Contents Part I Sample preparation basics 1 MECHANICAL/PHYSICAL METHODS OF CELL DISRUPTION AND TISSUE HOMOGENIZATION (Stanley Goldberg, unusual chapter format!!!!) 2 Bacteria and yeast cells disruption using lytic enzymes (Oriana Salazar) 3 Sample solubilization buffers for two-dimensional electrophoresis (Walter Weiss, unusual chapter format!!!!)4 Quantitation of protein in samples prepared for 2-D electrophoresis (Thomas Berkelman)5 Removal of Interfering Substances in Samples Prepared for 2-D Electrophoresis (Thomas Berkelman) 6 Protein Concentration by Hydrophilic Interaction Chromatography combined with Solid Phase Extraction (Ulrich Schneider) Part II Protein labeling techniques 7 Difference Gel Electrophoresis Based on Lys/Cys Tagging (Reiner Westermeier) 8 Isotope-coded two-dimensional maps: tagging with deuterated acrylamide and 2- vinylpyridine (Pier Giorgio Righetti, unusual chapter format!!!!)9 Stable Isotope Labeling by Amino acids in Cell culture (SILAC) (Albrecht Gruhler)10 ICPL - Isotope-coded protein label (Josef Kellermann)11 Radiolabeling for Two-Dimensional Gel Analysis (Hélian Boucherie) Part III Fractionation of proteins by chemical reagents and chromatography 12 Sequential extraction of proteins by chemical reagents (Stuart Cordwell) 13 Reducing sample complexity by RP-HPLC: beyond the tip of the protein expression iceberg (Gert van den Bergh)14 Enriching Basic and Acidic Rat Brain Proteins with Ion Exchange Mini Spin Columns Prior To Two-Dimensional Gel Electrophoresis (Ning Liu)15 Reducing the complexity of the Escherichia coli proteome by chromatography on reactive dye columns (Phil Cash)16 Reducing sample complexity in Proteomics by Chromatofocusing with simple buffer mixtures (Douglas D.Frey)17 Fractionation of Proteins by Immobilized Metal Affinity Chromatography(Qing-Yu He)18 Fractionation of proteins by heparin chromatography (Qing-Yu He) Part IV Fractionation of proteins by