A Genetic Approach to Plant Biochemistry

Variation
B. Somaclonal Variation
C. Gene Duplication
D. Ds Element Mutations in Adh1
E. Robertson's Mutator
VII. Approaches to the Mechanism of Adh Gene Regulation
A. DNA Sequence Comparisons
B. Mutations in Adh Which Affect Expression
VIII. In Vivo Expression of Adh
A. Attempts at Expression in Cereal Tissue Culture Cells
B. Testing Maize Adh Gene Activity in Dicotyledonous Plant Cells
C. Expression of Maize Adh in Animal Cells
IX. Conclusions
X. References

5 The Molecular Genetics of Higher Plant Nitrate Assimilation
I. Introduction
II. The Nitrate Assimilation Pathway
A. Nitrate Uptake
B. Nitrate Reductase
C. Nitrite Reductase
III. Genetics of Nitrate Assimilation
A. Introduction
B. Nitrate Uptake Mutations
C. Nitrate Reductase Mutations
D. Nitrite Reductase Mutations
E. Regulatory Alterations in Nitrate Assimilation
F. Conclusions
IV. Applied Aspects
A. Somatic Hybridisation
B. Cloning Nitrate Assimilation Genes
C. Plant Gene Transfer Systems
D. Whole Plant Studies
V. References

6 Plant Genetic Approaches to Symbiotic Nodulation and Nitrogen Fixation in Legumes
I. Introduction
II. A General Description of Legume Nodule Ontogeny
III. The Parasponia-Bradyrhizobium Symbiosis
IV. Biochemical and Molecular Analysis of Plant Functions
V. Gene-for-Gene Aspects of Nodulation
VI. Existing Plant Variation in Symbiotic Nitrogen Fixation
VII Existing Single Locus Variation for Nodulation-Nitrogen Fixation
VIII. Induced Mutation in Symbiotic Characters
A. Pea and Chickpea Mutants
B. Soybean Nodulation Mutants
IX. Conclusions
X. References

7 Endosperm Proteins
I. Introduction
II. Origin and Development of the Endosperm
III. Classification of the Major Endosperm Proteins
IV. Biochemical Complexity and Genetic Variation of Endosperm Proteins
V. Gene Mutations
A. Role in Plant Breeding
B. Role in Genetics
C. Role in Biochemistry and Molecular Biology
D. Role in the Food Industry
VI. Chromosome Mutations
VII. Conclusions
VIII. References

8 Molecular Approaches to Plant and Pathogen Genes
I. Introduction
II. A Molecular Approach to Gene-for-Gene Resistance
A. The Shotgun Method
B. Transposon Mutagenesis or Gene Tagging
III. The Role of Toxins in Plant Disease
IV. Conclusions
V. References

9 Gametophytic Gene Expression
I. Introduction
II. Overlap Between Sporophytic and Gametophytic Genotypes
III. Gametophytic Gene Expression and the Angiosperms
IV. The Influence of Haploid Genotype on Pollen Size
V. Time of Gene Expression in Pollen
VI. Methods of Haploid Selection
VII. Gametophytic Gene Expression and the Style
VIII. Gene Expression in the Megagametophyte
IX. Conclusions
X. References

10 Auxotroph Isolation In Vitro
I. Introduction
A. Some Preliminary Comments
B. Why Auxotrophs?
C. The Characteristics, Advantages and Disadvantages of In vitro Systems for Mutant Isolation
D. Species Suitable for Auxotroph Isolation
II. Mutagenesis
III. Methods of Selection
A. Conditions and Media
B. Positive Selection
C. Negative Selection
IV. Phenotypes
A. Amino-Acid, Vitamin and Purine Auxotrophs
B. Nitrate Reductase Mutants
C. Temperature-Sensitive Mutations
V. Fusion and Transformation
VI. Conclusions and Future Prospects
VII. References