Activity-Based Proteomics
Methods and Protocols
(Sprache: Englisch)
This volume focuses on explorative activity-based proteomics, biomedical applications of activity-based proteomics, and chemical strategies in activity-based proteomics providing a concise overview of activity-based protein profiling. Written in the highly...
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Klappentext zu „Activity-Based Proteomics “
This volume focuses on explorative activity-based proteomics, biomedical applications of activity-based proteomics, and chemical strategies in activity-based proteomics providing a concise overview of activity-based protein profiling. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Activity-Based Proteomics: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Inhaltsverzeichnis zu „Activity-Based Proteomics “
Activity-Based Protein Profiling - From Chemical Novelty to Biomedical Stalwart.- A Quantitative Mass-Spectrometry Platform to Monitor Changes in Cysteine Reactivity.- Activity-Based Protein Profiling with Natural Product-Derived Chemical Probes in Human Cell Lysates.- Inhibitor Discovery by Convolution Activity-Based Protein Profiling.- Activity-Based Protein Profiling in Bacteria.- Activity-Based Lipid Esterase Profiling of M. Bovis BCG at Different Metabolic States Using Tetrahydrolipstatin (THL) as Bait.- Comparative Activity-Based Flavin-Dependent Oxidase Profiling.- Activity-Dependent Photoaffinity Labelling of Metalloproteases.- Profiling the Activity of Deubiquitinating Enzymes using Chemically Synthesized Ubiquitin-Based Probes.- Profiling Protein Sumoylation using Biochemical and Mass Spectrometry-Based Research Tools.- Live Cell Imaging and Profiling of Cysteine Cathepsin Activity using a Quenched Activity-Based Probe.- Competitive Activity-Based Protein Profiling Of Serine Hydrolases: A Case Study on DAGL-Alpha.- A Protocol for Protein Profiling using Chemoselective Cleavable Linker Probes in Semi-Permeabilized Cells.- Cleavable Linkers in Chemical Proteomics Applications.- Two-Step Activity-Based Protein Profiling With the Proteasome System as Model of Study.
Bibliographische Angaben
- 2016, 1st ed. 2017, XI, 222 Seiten, 18 farbige Abbildungen, Maße: 18,7 x 26,1 cm, Gebunden, Englisch
- Herausgegeben: Herman S. Overkleeft, Bogdan I. Florea
- Verlag: Springer, Berlin
- ISBN-10: 1493964372
- ISBN-13: 9781493964376
- Erscheinungsdatum: 25.10.2016
Sprache:
Englisch
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