Adenovirus Methods and Protocols 1

TABLE OF CONTENTSVol. I Topics: Adenoviruses and vectors, quantitation, and animal models1. Manipulation of Early Region 4Julie Boyer and Gary Ketner2. Isolation, Growth, and Purification of Defective Adenovirus Deletion MutantsGary Ketner and Julie Boyer3. Construction of Adenovirus Type 5 Early Region 1 (E1) and 4 (E4) Virus MutantsPeter Groitl and Thomas Dobner4. Construction of Mouse Adenovirus Type 1 MutantsAngela N. Cauthen, Amanda R. Welton, and Katherine R. Spindler5. Generation of Recombinant Adenovirus using E.coli BJ5183 Recombination SystemP. Seshidhar Reddy, Shanthi Ganesh, Lynda Hawkins, Neeraja Idamakanti6. Production and Release Testing of Ovine AtadenovirusVectors Gerald W. Both, Fiona Cameron, Anne Collins, Linda J. Lockett, Jan Shaw 7. Construction of Capsid Modified Recombinant Bovine Adenovirus Type 3Alexander N. Zakhartchouk, Qiaohua Wu, Suresh K. Tikoo8. Adenovirus Capsid Chimeras: Fiber Terminal Exon Insertions / Gene Replacements in the MLTUJason Gall, John Schoggins, and Erik Falck-Pedersen9. Temperature-Sensitive Replication-Competent Adenovirus shRNA Vectors to Study Cellular Genes in Virus-Induced ApoptosisT. Subramanian and G. Chinnadurai10. Evaluating Apoptosis in Tumor Cells Infected with Adenovirus Expressing p53 Tumor Suppressor Gene Elizabeth Perron, M. Behzad Zafar, Amanda Pister, Zhen-Guo Wang, Jun Tian and Prem Seth11. Growth and Purification of Enteric Adenovirus Type 40Vivien Mautner12. Immunocompetent, Semi-permissive Cotton Rat Tumor Model for the Evaluation of Oncolytic Adenoviruses Karoly Toth, Jacqueline F. Spencer, William S.M. Wold13. The Use of the Syrian Hamster as an Animal Model for Oncolytic Adenovirus VectorsMaria A. Thomas, Jacqueline F. Spencer, and William S. M. Wold14. A Real-Time PCR Method to Rapidly Titer Adenovirus StocksMaria A. Thomas, Drew L. Lichtenstein, Peter Krajcsi, and William S. M. Wold15. Detection and Quantitation of Subgroup C Adenovirus DNA in Human Tissue Samples by Real-Time PCRC.T.

"...provdes some good advice on using and growing adenovirus vectors." -Doody's Book Review, Weighted Numerical Score: 62 - 2 Stars Reviews of the First edition:"This book caters quite well to those who simply want to grow adenoviruses, assay them, and use them. . .a very comprehensive methods book that will be a laboratory standard for years to come."-Microbiology Today". . .this book constitutes an excellent reference text . . .this book is replete with useful information for both the virologist and the non-virologist who is interested in working with adenoviruses."-FEBS Letters