Advanced Protocols in Oxidative Stress I

Part I. Reactive Oxygen and Nitrogen Techniques 1. Novel Designed Probes for the Characterization of Oxidative Stress in Biological Fluids, Cell and Tissues Jacob Vaya 2. A Rapid and Selective Mass Spectroscopic Method for the Identification of Nitrated Proteins Angela Amoresano, Giovanni Chiappetta, Piero Pucci and Gennaro Marino 3. An Easy and Reliable Automated Method to Estimate Oxidative Stress in the Clinical Setting Cristina Vassalle 4. Correlative Transmission Microscopy: Cytochemical Localization and Immunocytochemical Localization in Studies of Oxidative and Nitrosative Stress E. Ann Ellis 5. Detection of Specifically Oxidized Apolipoproteins in Oxidized HDL Xiao Suo Wang and Roland Stocker 6. High Performance Liquid Chromatography / Electron Spin Resonance / Mass Spectroscopy Analysis of Lipid-Derived Radicals Hideo Iwahashi 7. EPR Spin-Trapping and Nano LC MS/MS Techniques for DEPMPO/ OOH and Immunospin-trapping with Anti-DMPO Antibody in Mitochondrial Electron Transfer System Yeong-Renn Chen 8 Determination of High Mitochondrial Membrane Potential in Spermatazoa loaded with the Mitochondrial Probe 5, 5', 6, 6'- tetrachloro-1, 1', 3, 3'-tetraethylbenzimazoyolyl-carbocyanine iodide ( JC-! ) and for Using Fluorescence-activated Flow Cytometry H. David Guthrie and Glenn R. Welch 9. TEMPO Probes for Evaluating Oxidative Stress on the Cell Membrane and Mitochondria Hidehiko Nakagawa and Naoki Miyata 10. Zymographical Techniques for Detection of Matrix Metallproteinases Shinichi Iwai, Takako Nakanishi-Ueda , Donald Armstrong and Katsuji Oguchi 11. The Use of in vivo Microanalysis Techniques to Detect Exracellular ROS in Resting and Contracting Skeletal Muscle Graeme L. Close and Malcolm J. Jackson 12. Cell-free Antibody Capture Method for Analysis of Detergent-Resistant Membrane Rafts Anil Bamezai and Colleen Kennedy 13. Determination of Acrolein by High Voltage Capillary Electrophoresis from Oxidized Fatty Acids Rafael Medina-Navarro Part II. Antioxidant