Animal Cell Biotechnology

High-Throughput Synchronization of Mammalian Cell Cultures by Spiral Microfluidic Sorter.- Insect Cell Line Development Using Flp-Mediated Cassette Exchange Technology.- Scalable Transient Gene Expression in Adherent Mammalian Cells Using Polyethylenimine.- Scalable Transient Protein Expression.- DNA Profiling and Characterisation of Animal Cell Lines.- Tools for High-Throughput Process and Medium Optimization.- Designing Media for Animal Cell Culture - CHO Cells, The Industrial Standard.- Feed Optimization in Fed-Batch Culture.- Screening and Optimization of Chemically-Defined Media and Feeds with Integrated and Statistical Approaches.- Evaluation of Solid and Porous Microcarriers for Cell Growth and Production of Recombinant Proteins.- Microbioreactors and Scale-Down Models: Growth of CHO Cells Using the Pall Micro24 MicroReactor System.- Monitoring Cell Growth, Viability and Apoptosis.- Metabolic Flux Estimation in Mammalian Cell Cultures.- Quenching Methods for the Analysis of Intracellular Metabolites.- NMR Methods for Metabolomics of Mammalian Cell Culture Bioreactors.- 1H-NMR Protocol for Exometabolome Analysis of Cultured Mammalian Cells.-Microfluidic Perfusion Culture.- Disposable Bioreactors for Inoculum Production and Protein Expression.- Configuration of Bioreactors.- Process Analytical Technology (PAT) in Insect and Mammalian Cell Culture Processes - Dielectric Spectroscopy and Focused Beam Reflectance Measurement (FBRM).- Cell Assessment by Atline Microscopy.- Seed Train Optimization for Cell Culture.- Vaccine Production - Upstream Processing with Adherent or Suspension Cell Lines.- Efficient Processes for Protein Expression Using Recombinant Baculovirus Particles.- Chromatographic Techniques in the Downstream Processing of Proteins in Biotechnology.- Virus Separation Using Membranes.