Antigen Processing

Preface... Table of Contents... Contributing Authors... 1. Purification of Large Cytosolic Proteases for In Vitro Assays: 20S and 26S Proteasomes Stefan Tenzer and Hansjörg Schild 2. Analysis of Proteasome Generated Antigenic Peptides by Mass Spectrometry Kathrin Textoris-Taube, Ulrike Kuckelkorn, Christin Keller, and Peter-M. Kloetzel 3. Trimming of MHC Class I Ligands by ERAP Aminopeptidases Mirjana Weimershaus, Irini Evnouchidou, Peter van Endert, and Marlene Bouvier 4. Peptide Trimming for MHC Class I Presentation by Endoplasmic Reticulum Aminopeptidases Mirco Compagnone and Doriana Fruci 5. Testing the Impact of Protease Inhibitors in Antigen Presentation Assays Michael Basler and Marcus Groettrup 6. In Vitro Studies of MHC Class I Peptide Loading and Exchange Marlene Bouvier 7. Measuring Synthesis and Degradation of MHC Class I Molecules Simon J. Powis and Antony N. Antoniou 8. Biochemical Analysis of Naturally Processed Antigenic Peptides Presented by MHC Class I Molecules Takayuki Kanaseki and Nilabh Shastri 9. A SIINFEKL Based System to Measure MHC Class I Antigen Presentation Efficiency and Kinetics Devin Dersh, Jonathan W. Yewdell, and Jiajie Wei 10. Purification and Identification of Naturally Presented MHC Class I and II Ligands Annika Nelde, Daniel J. Kowalewski, and Stefan Stevanovic 11. Identification of MHC Ligands and Establishing MHC Class I Peptide Motifs Michael Ghosh, Moreno Di Marco, and Stefan Stevanovic 12. Quantitating MHC Class I Ligand Production and Presentation using TCR-Like Antibodies Brian P. Dolan 13. Identifying Antigens Recognized by Cytolytic T Lymphocytes on Tumors Nathalie Vigneron, Vincent Stroobant, Wenbin Ma, Alexandre Michaux, and Benoît J. Van den Eynde 14. A Flow Cytometry-Based Approach to Unravel Viral Interference with the MHC Class I Antigen Processing and Presentation Pathway Patrique Praest, Hendrik de Buhr, and Emmanuel J.H.J. Wiertz 15. Evaluating CD8+ T Cell Responses In Vitro François A. Lemonnier 16.