Axon Growth and Regeneration

Axon Regeneration: What Needs to Be Overcome?.- Analysis of Calcium Signals in Steering Neuronal Growth Cones In Vitro.- The Use of Fluorescence Resonance Energy Transfer (FRET) to Measure Axon Growth and Guidance Related Intracellular Signaling in Live Dorsal Root Ganglia Neuronal Growth Cones.- Neurite Outgrowth and Growth Cone Collapse Assays to Assess Neuronal Responses to Extracellular Cues.- Quantitative Assessment of Neurite Outgrowth in Mouse Retinal Explants.- Growth Cone Collapse Assay.- Axon Length Quantification Microfluidic Culture Platform for Growth and Regeneration Study.- Organotypic Slice Co-Culture Systems to Study Axon Regeneration in the Dopaminergic System Ex Vivo.- Monitoring Neuron and Astrocyte Interactions with a 3D Cell Culture System.- Targeting Inhibitory Chondroitin Sulphate Proteoglycans to Promote Plasticity After Injury.- Polymeric Biomaterials for Nerve Regeneration: Fabrication and Implantation of a Biodegradable Nerve Guide.- A Highly Reproducible Mouse Model of Compression Spinal Cord Injury.- Using Templated Agarose Scaffolds to Promote Axon Regeneration Through Sites of Spinal Cord Injury.- In Vivo Electroporation of Adult Mouse Sensory Neurons for Studying Peripheral Axon Regeneration.- Assessing Motor Outcome and Functional Recovery Following Nerve Injury.- The Use of an Adeno-Associated Viral Vector for Efficient Bicistronic Expression of Two Genes in the Central Nervous System.- Application of Tissue Clearing and Light Sheet Fluorescence Microscopy to Assess Optic Nerve Regeneration in Unsectioned Tissues.- Time-Lapse In Vivo Imaging of Dorsal Root Nerve Regeneration in Mice.- Using Manganese-Enhanced MRI to Assess Optic Nerve Regeneration.

From the reviews:"This is a 'how to' manual of protocols, parameters, and cell biology methods for research studies of neurite outgrowth assays. ... The authorship is very good and interesting to neurobiologists, cell culture experts, and neurology-neurosurgery researchers. ... I highly recommend this book for all neurobiologists with interests in cell and slice physiology." (Joseph J. Grenier, Amazon.com, June, 2014)