Basic Cloning Procedures
(Sprache: Englisch)
This manual introduces the reader to bacic methods used in the isolation, cloning and analysis of genetic material. The protocols include RT-PCR amplification, gene cloning, hybridization analysis and sequencing of nucleic acids, PCR-based site-specific...
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Klappentext zu „Basic Cloning Procedures “
This manual introduces the reader to bacic methods used in the isolation, cloning and analysis of genetic material. The protocols include RT-PCR amplification, gene cloning, hybridization analysis and sequencing of nucleic acids, PCR-based site-specific mutagenesis, analysis of protein DNA-specific interaction, cell-free protein synthesis and product electrophoretic and immunological analysis. Each protocol includes short background information, a detailed description of the necessary materials, step-by-step procedures, a troubleshooting guide and useful practical hints.
Inhaltsverzeichnis zu „Basic Cloning Procedures “
1 cDNA Synthesis and Cloning.- 2 DNA Sequencing.- 3 RNA In Vitro Synthesis by Phage T7 DNA-Dependent RNA Polymerase.- 4 PCR-Based Site-Specific Mutagenesis.- 5 Analysis of Specific Protein-DNA Interactions.- 6 Gel Electrophoresis, Transfer and Hybridization of Nucleic Acids.- 7 Immunological Methods for Analysis of Recombinant Proteins.- 8 Protein Synthesis in Cell-Free Systems.
Bibliographische Angaben
- 2012, VII, 169 Seiten, Maße: 15,5 x 23,5 cm, Kartoniert (TB), Englisch
- Herausgegeben: Valdis Berzins
- Verlag: Springer, Berlin
- ISBN-10: 364248977X
- ISBN-13: 9783642489778
Sprache:
Englisch
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